Product Name
ACTG2, siRNA
Full Product Name
ACTG2 siRNA (Human)
Product Synonym Names
ACTA3; ACTL3; ACTSG; Actin gamma-enteric smooth muscle; Alpha-actin-3; Gamma-2-actin; Smooth muscle gamma-actin
Product Gene Name
ACTG2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P63267
Specificity
ACTG2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ACTG2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ACTG2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ACTG2 sirna
siRNA to inhibit ACTG2 expression using RNA interference
Applications Tested/Suitable for ACTG2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ACTG2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001186822.1
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NCBI GenBank Nucleotide #
NM_001199893.1
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UniProt Primary Accession #
P63267
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UniProt Secondary Accession #
P12718; Q504R1; Q6FI22; B2R7E7; B4E315; D6W5H8; E9PG30[Other Products]
UniProt Related Accession #
P63267[Other Products]
Molecular Weight
37,083 Da
NCBI Official Full Name
actin, gamma-enteric smooth muscle isoform 2
NCBI Official Synonym Full Names
actin, gamma 2, smooth muscle, enteric
NCBI Official Symbol
ACTG2 [Similar Products]
NCBI Official Synonym Symbols
ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG
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NCBI Protein Information
actin, gamma-enteric smooth muscle
UniProt Protein Name
Actin, gamma-enteric smooth muscle
UniProt Synonym Protein Names
Alpha-actin-3; Gamma-2-actin; Smooth muscle gamma-actin
UniProt Gene Name
ACTG2 [Similar Products]
UniProt Synonym Gene Names
ACTA3; ACTL3; ACTSG [Similar Products]
UniProt Entry Name
ACTH_HUMAN
NCBI Summary for ACTG2
Actins are highly conserved proteins that are involved in various types of cell motility and in the maintenance of the cytoskeleton. Three types of actins, alpha, beta and gamma, have been identified in vertebrates. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. This gene encodes actin gamma 2; a smooth muscle actin found in enteric tissues. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Based on similarity to peptide cleavage of related actins, the mature protein of this gene is formed by removal of two N-terminal peptides.[provided by RefSeq, Dec 2010]
UniProt Comments for ACTG2
ACTG2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family.
Protein type: Motility/polarity/chemotaxis; Contractile; Cytoskeletal
Chromosomal Location of Human Ortholog: 2p13.1
Cellular Component: extracellular space; cytoskeleton; cytosol
Molecular Function: ATP binding
Biological Process: muscle contraction
Disease: Visceral Myopathy
Research Articles on ACTG2
1. ACTG2 encodes g2 enteric actin and is the first gene to be clearly associated with Megacystis-microcolon-intestinal hypoperistalsis syndrome, suggesting an important role for contractile proteins in enteric smooth muscle disease.
Precautions
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Disclaimer
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