AOS1, Antibody

activator of SUMO1; AOS1; FLJ3091; HSPC140; SAE1; sentrin/SUMO-activating protein AOS1; SUA1; SUMO-1 activating enzyme E1 N subunit; SUMO-1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; SUMO1 activating enzyme subunit 1; Ubiquitin-like 1

For Research Use Only. Not for use in diagnostic procedures.

AOS1 antibody detects endogenous levels of total AOS1

Affinity-chromatography

In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at-20 degree C for 1 year

Small volumes of anti-AOS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

UBLE1A The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2. Belongs to the ubiquitin-activating E1 family. Heterodimer of SAE1 and SAE2. The complex binds SUMO proteins via SAE2.

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

WB: 1:500~1:3000
IF/ICC: 1:100~1:500

Western blot analysis on 293 cell lysate using AOS1 Antibody

38kDa

SUMO1 activating enzyme subunit 1

SUMO-activating enzyme subunit 1

SUMO-activating enzyme subunit 1

AOS1; SUA1; UBLE1A  [Similar Products]

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

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