Product Name
alpha Tubulin (TUBA1B), Polyclonal Antibody
Full Product Name
alpha Tubulin Antibody: Dylight 350
Product Synonym Names
Rabbit Anti-Human alpha Tubulin Antibody Polyclonal; Alpha-tubulin ubiquitous Antibody; Alpha tubulin ubiquitous Antibody; Tubulin alpha 1B chain Antibody; Tubulin alpha ubiquitous Antibody; Tubulin K-alpha-1 Antibody; TBA1B_HUMAN Antibody; K alpha 1 Antibody; Tubulin alpha ubiquitous chain Antibody; Tubulin alpha 1B Antibody; TUBA1B Antibody; Tubulin alpha-1B chain Antibody; Tubulin alpha-ubiquitous chain Antibody; Tubulin K alpha 1 Antibody
Product Gene Name
anti-TUBA1B antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P68363
Specificity
Detects ~50 kDa.
Purity/Purification
Peptide Affinity Purified
Form/Format
PBS, 50% Glycerol, 0.09% Sodium Azide
Concentration
1 mg/ml (lot specific)
Immunogen
Synthetic peptide of Human alpha-Tubulin
Cellular Localization
Cytoplasm; Cytoskeleton
Certificate of Analysis
A 1:1000 dilution was sufficient for detection of alpha-Tubulin in 15 ug of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Product Notes
Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, Förster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Preparation and Storage
Store at-20 degree C.
Other Notes
Small volumes of anti-TUBA1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-TUBA1B antibody
Tags and Cell Markers; Organelle Markers; Cell Signaling; Cytoskeleton; Microtubules
Applications Tested/Suitable for anti-TUBA1B antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-TUBA1B antibody
WB: 1:1000
ICC/IF: 1:100
Immunocytochemistry (ICC) of anti-TUBA1B antibody
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-alpha Tubulin Polyclonal Antibody. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-alpha Tubulin Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 50.1 kDa.
NCBI/Uniprot data below describe general gene information for TUBA1B. It may not necessarily be applicable to this product.
NCBI Accession #
P68363.1
[Other Products]
UniProt Primary Accession #
P68363
[Other Products]
UniProt Related Accession #
P68363[Other Products]
NCBI Official Full Name
Tubulin alpha-1B chain
NCBI Official Synonym Full Names
tubulin alpha 1b
NCBI Official Symbol
TUBA1B [Similar Products]
NCBI Official Synonym Symbols
K-ALPHA-1
[Similar Products]
NCBI Protein Information
tubulin alpha-1B chain
UniProt Protein Name
Tubulin alpha-1B chain
UniProt Synonym Protein Names
Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain
UniProt Gene Name
TUBA1B [Similar Products]
UniProt Entry Name
TBA1B_HUMAN
UniProt Comments for TUBA1B
TUBA1B: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 12q13.12
Cellular Component: microtubule; cytoplasmic microtubule
Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton; ubiquitin protein ligase binding; double-stranded RNA binding; structural molecule activity
Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; cell division; cytoskeleton-dependent intracellular transport; microtubule cytoskeleton organization and biogenesis; microtubule-based process
Research Articles on TUBA1B
1. Data show that retinal endothelial cells (HREC) treated with low molecular weight fraction of commercial 5% human serum albumin (LMWF5A) exhibit a rapid increase in the amount and distribution of acetylated alpha-tubulin.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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