Full Product Name
FKHR Antibody
Product Synonym Names
FKHR; forkhead box O1A (FKHR); Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; FOXO1
Product Gene Name
anti-FKHR antibody
[Similar Products]
Product Synonym Gene Name
FOXO1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q12778
Species Reactivity
Human, Mouse
Specificity
FKHR Antibody detects endogenous levels of total FKHR protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human FKHR.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-FKHR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-FKHR antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-FKHR antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:20000
Immunohistochemistry (IHC) of anti-FKHR antibody
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using FKHR Antibody. The picture on the right is blocked with the synthesized peptide.
Western Blot (WB) of anti-FKHR antibody
Western blot analysis of lysates from HeLa cells, treated with EGF+Serum, using FKHR Antibody. The lane on the right is blocked with the synthesized peptide.
NCBI/Uniprot data below describe general gene information for FKHR. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002006.2
[Other Products]
NCBI GenBank Nucleotide #
NM_002015.3
[Other Products]
UniProt Primary Accession #
Q12778
[Other Products]
UniProt Secondary Accession #
O43523; Q5VYC7; Q6NSK6[Other Products]
UniProt Related Accession #
Q12778[Other Products]
NCBI Official Full Name
forkhead box protein O1
NCBI Official Synonym Full Names
forkhead box O1
NCBI Official Symbol
FOXO1 [Similar Products]
NCBI Official Synonym Symbols
FKH1; FKHR; FOXO1A
[Similar Products]
NCBI Protein Information
forkhead box protein O1
UniProt Protein Name
Forkhead box protein O1
UniProt Synonym Protein Names
Forkhead box protein O1A; Forkhead in rhabdomyosarcoma
UniProt Gene Name
FOXO1 [Similar Products]
UniProt Synonym Gene Names
FKHR; FOXO1A [Similar Products]
NCBI Summary for FKHR
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]
UniProt Comments for FKHR
Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose tissue. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner. Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling ().
Research Articles on FKHR
1. Low FOXO1 expression is associated with hepatocellular carcinoma.
Precautions
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Disclaimer
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