Product Name
CLCNKB, siRNA
Full Product Name
CLCNKB siRNA (Rat)
Product Synonym Names
Chloride channel protein ClC-Kb; Chloride channel Kb; ClC-K2
Product Gene Name
CLCNKB sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P51802
Specificity
CLCNKB siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CLCNKB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CLCNKB sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CLCNKB sirna
siRNA to inhibit CLCNKB expression using RNA interference
Applications Tested/Suitable for CLCNKB sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CLCNKB. It may not necessarily be applicable to this product.
NCBI Accession #
NP_775126.1
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NCBI GenBank Nucleotide #
NM_173103.1
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UniProt Primary Accession #
P51802
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UniProt Related Accession #
P51802[Other Products]
Molecular Weight
69,211 Da
NCBI Official Full Name
chloride channel protein ClC-Kb
NCBI Official Synonym Full Names
chloride channel, voltage-sensitive Kb
NCBI Official Symbol
Clcnkb [Similar Products]
NCBI Official Synonym Symbols
Clck2; ClC-K2L; Clcnk1l
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NCBI Protein Information
chloride channel protein ClC-Kb
UniProt Protein Name
Chloride channel protein ClC-Kb
UniProt Synonym Protein Names
ClC-K2
Protein Family
Chloride channel protein
UniProt Gene Name
Clcnkb [Similar Products]
UniProt Synonym Gene Names
Chloride channel Kb [Similar Products]
UniProt Entry Name
CLCKB_RAT
NCBI Summary for CLCNKB
This gene is a member of the CLC family of voltage-gated chloride channels. The gene is located adjacent to a highly similar chloride channel gene on chromosome 5. This gene is syntenic with human CLCNKA (geneID:1187).[provided by RefSeq, Sep 2009]
UniProt Comments for CLCNKB
CLCNKB: Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms. Defects in CLCNKB are the cause of Bartter syndrome type 3 (BS3); also known as classic Bartter syndrome. It is an autosomal recessive form of often severe intravascular volume depletion due to renal salt-wasting associated with low blood pressure, hypokalemic alkalosis, hypercalciuria, and normal serum magnesium levels. Defects in CLCNKB are a cause of Bartter syndrome type 4B (BS4B). A digenic, recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. Bartter syndrome type 4B is associated with sensorineural deafness. Belongs to the chloride channel (TC 2.A.49) family. CLCNKB subfamily.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter, ion channel; Transporter
Cellular Component: Golgi membrane; plasma membrane; integral to membrane
Molecular Function: chloride channel activity; metal ion binding; voltage-gated chloride channel activity
Biological Process: transepithelial chloride transport; chloride transport
Research Articles on CLCNKB
1. In salt-sensitive hypertension, there is a strong upregulation, both of NCC and ClC-K along the distal convoluted tubules, which explains the persistence of hypertension.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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