Product Name
KCNE1, siRNA
Full Product Name
KCNE1 siRNA (Rat)
Product Synonym Names
Potassium voltage-gated channel subfamily E member 1; Delayed rectifier potassium channel subunit IsK; IKs producing slow voltage-gated potassium channel subunit beta Mink; Minimal potassium channel
Product Gene Name
KCNE1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P15383
Specificity
KCNE1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat KCNE1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of KCNE1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
KCNE1 sirna
siRNA to inhibit KCNE1 expression using RNA interference
Applications Tested/Suitable for KCNE1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for KCNE1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_037105.1
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NCBI GenBank Nucleotide #
NM_012973.1
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UniProt Primary Accession #
P15383
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UniProt Related Accession #
P15383[Other Products]
Molecular Weight
14,699 Da
NCBI Official Full Name
potassium voltage-gated channel subfamily E member 1
NCBI Official Synonym Full Names
potassium channel, voltage-gated Isk-related subfamily E regulatory beta subunit 1
NCBI Official Symbol
Kcne1 [Similar Products]
NCBI Official Synonym Symbols
m; Isk
[Similar Products]
NCBI Protein Information
potassium voltage-gated channel subfamily E member 1
UniProt Protein Name
Potassium voltage-gated channel subfamily E member 1
UniProt Synonym Protein Names
Delayed rectifier potassium channel subunit IsK; IKs producing slow voltage-gated potassium channel subunit beta Mink; Minimal potassium channel
Protein Family
Potassium voltage-gated channel subfamily
UniProt Gene Name
Kcne1 [Similar Products]
UniProt Entry Name
KCNE1_RAT
NCBI Summary for KCNE1
beta subunit of the voltage-gated potassium channel complex; involved in modulating channel kinetics [RGD, Feb 2006]
UniProt Comments for KCNE1
KCNE1: Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNQ1/KVLQT1 is proposed to form the slowly activating delayed rectifier cardiac potassium (IKs) channel. The outward current reaches its steady state only after 50 seconds. Assembled with KCNH2/HERG may modulate the rapidly activating component of the delayed rectifying potassium current in heart (IKr). Associates with KCNQ1/KVLQT1 and KCNH2/HERG. Expressed in heart, lung, kidney, testis, ovaries, small intestine, peripheral blood leukocytes. Not detected in pancreas, spleen, prostate and colon. Restrictively localized in the apical membrane portion of epithelial cells. Belongs to the potassium channel KCNE family.
Protein type: Channel, potassium; Membrane protein, integral
Cellular Component: Golgi apparatus; voltage-gated potassium channel complex; cell surface; endoplasmic reticulum; lysosome; apical plasma membrane; plasma membrane; Z disc
Molecular Function: protein binding; voltage-gated potassium channel activity; potassium channel regulator activity; telethonin binding; protein heterodimerization activity; delayed rectifier potassium channel activity
Biological Process: protein amino acid O-linked glycosylation; heart contraction; epithelial cell maturation; male gonad development; protein amino acid N-linked glycosylation
Research Articles on KCNE1
1. KCNE1 and KCNE2, auxiliary subunits of voltage-gated potassium channels, undergo sequential cleavage mediated by either alpha-secretase and presenilin(PS)/gamma-secretase or BACE1 and PS/gamma-secretase in cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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