CRBN, Polyclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Affinity Purified

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-CRBN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This is a rabbit polyclonal antibody against CRBN. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: This gene encodes a protein related to the Lon protease protein family. In rodents and other mammals this gene product is found in the cytoplasm localized with a calcium channel membrane protein, and is thought to play a role in brain development. Mutatio

Polyclonal; Developmental Biology; Various; Membrane & Traffic;

Western Blot (WB)

Host: Rabbit
Target Name: CRBN
Sample Tissue: Human DLD1 Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: CRBN
Sample Tissue: Human HCT15
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: CRBN
Sample Tissue: Human Liver Tumor
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: CRBN
Sample Tissue: Human Ovary Tumor
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: CRBN
Sample Type: RPMI-8226 Whole Cell lysates
Antibody Dilution: 0.5ug/ml

WB Suggested Anti-CRBN Antibody Titration: 0.2-1 ug/ml
Positive Control: COLO205 cell lysate

50kDa

cereblon

protein cereblon

Protein cereblon

CRBN_HUMAN

This gene encodes a protein related to the Lon protease protein family. In rodents and other mammals this gene product is found in the cytoplasm localized with a calcium channel membrane protein, and is thought to play a role in brain development. Mutations in this gene are associated with autosomal recessive nonsyndromic cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

CRBN: Component of some DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. In the complex, may act as a substrate receptor. Regulates the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Defects in CRBN are the cause of mental retardation autosomal recessive type 2A (MRT2A). MRT2A patients display mild mental retardation with a standard IQ ranged from 50 to 70. IQ scores are lower in males than females. Developmental milestones are mildly delayed. There are no dysmorphic or autistic features. Non-syndromic mental retardation patients do not manifest other clinical signs. Belongs to the CRBN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3p26.2

Cellular Component: membrane; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; ATP-dependent peptidase activity; metal ion binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination; negative regulation of protein homooligomerization

Disease: Mental Retardation, Autosomal Recessive 2

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.