5-hydroxytryptamine receptor 7 (HTR7), ELISA Kit

5-HT7; 5-hydroxytryptamine receptor 7; serotonin 5-HT-7 receptor

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Mouse HTR7. No significant cross-reactivity or interference between Mouse HTR7 and analogues was observed.

Store at 2-8 degree C.

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Small volumes of HTR7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS287599 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-hydroxytryptamine receptor 7 (HTR7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HTR7. The ELISA analytical biochemical technique of the MBS287599 kit is based on HTR7 antibody-HTR7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HTR7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HTR7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Description: This assay employs a two-site sandwich ELISA to quantitate HTR7 in samples. An antibody specific for HTR7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HTR7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HTR7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HTR7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: The receptor is a member of the GPCR superfamily of cell surface receptors and is activated by the neurotransmitter serotonin (5-HT) The 5-HT7 receptor is coupled to Gs (stimulates the production of the intracellular signaling molecule cAMP) and is expressed in a variety of human tissues, particularly in the brain, the gastrointestinal tract, and in various blood vessels. This receptor has been a drug development target for the treatment of several clinical disorders. The 5-HT7 receptor is encoded by the intron containing HTR7 gene, which in humans is transcribed into 3 different splice variants.In humans, the neurotransmitter serotonin (5-HT) plays an important role in various cognitive and behavioral functions. The serotonin receptor encoded by the HTR7 gene belongs to the superfamily of G protein-coupled receptors (GPCRs).

49,916 Da

5-hydroxytryptamine (serotonin) receptor 7

5-hydroxytryptamine receptor 7

5-hydroxytryptamine receptor 7

5-HT-7; 5-HT7  [Similar Products]

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.

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