Full Product Name
CRBN siRNA (Human)
Product Synonym Names
Protein cereblon
Product Gene Name
CRBN sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q96SW2
Specificity
CRBN siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CRBN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CRBN sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CRBN sirna
siRNA to inhibit CRBN expression using RNA interference
Applications Tested/Suitable for CRBN sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CRBN. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001166953.1
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NCBI GenBank Nucleotide #
NM_001173482.1
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UniProt Primary Accession #
Q96SW2
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UniProt Secondary Accession #
Q6AI62; Q6NVZ0; Q9UHW4; B2R6H4; C9IZA9; C9JAH6[Other Products]
UniProt Related Accession #
Q96SW2[Other Products]
Molecular Weight
50,475 Da
NCBI Official Full Name
protein cereblon isoform 2
NCBI Official Synonym Full Names
cereblon
NCBI Official Symbol
CRBN [Similar Products]
NCBI Official Synonym Symbols
MRT2; MRT2A
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NCBI Protein Information
protein cereblon
UniProt Protein Name
Protein cereblon
Protein Family
Protein cereblon
UniProt Gene Name
CRBN [Similar Products]
UniProt Entry Name
CRBN_HUMAN
NCBI Summary for CRBN
This gene encodes a protein related to the Lon protease protein family. In rodents and other mammals this gene product is found in the cytoplasm localized with a calcium channel membrane protein, and is thought to play a role in brain development. Mutations in this gene are associated with autosomal recessive nonsyndromic mental retardation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
UniProt Comments for CRBN
CRBN: Component of some DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. In the complex, may act as a substrate receptor. Regulates the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Defects in CRBN are the cause of mental retardation autosomal recessive type 2A (MRT2A). MRT2A patients display mild mental retardation with a standard IQ ranged from 50 to 70. IQ scores are lower in males than females. Developmental milestones are mildly delayed. There are no dysmorphic or autistic features. Non-syndromic mental retardation patients do not manifest other clinical signs. Belongs to the CRBN family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 3p26.2
Cellular Component: membrane; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; metal ion binding; ATP-dependent peptidase activity
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination; negative regulation of protein homooligomerization
Disease: Mental Retardation, Autosomal Recessive 2
Research Articles on CRBN
1. The study presents the crystal structure of human CRBN bound to DDB1 and the drug lenalidomide.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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