Agouti, Protein

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 20; NC_000020.10 (32829188..32857150). Location: 20q11.2-q12

Synthetic peptied consisting of 21aa near the N-terminus of mouse Agouti. No sequence homology is seen with AGRP, tubby, tub, TULP1 and TULP2. Species Sequence Homology

Purified
Purified

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of ASIP protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Control peptide for A1059-58 (anffinity purified antibody) and A1059-59B (antiserum).

Several common diseases such as type II diabetes, hypertension, cardiovascular diseases, hyperlipidemia, and some cancers are associated with obesity. An abnormal increase in body fat relative to lean tissue mass has been used as an indicator of obesity. High fat diet, certain environmental factors, and genetic linkage are the primary causes of obesity. In order to understand the genetic basis of obesity, several monogenic murine obesity models have been characterized including obese (Ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub). More recently, Tub, the human homolog of mouse Tub, TULP1 & TULP2 (for Tubby Like Proteins) and Agouti related protein (AGRP) have been cloned. The obesity associated with Ay mice may be due to ectopic expression of a secreted protein Agouti. Agouti protein (132aa in human) is normally expressed in skin. Its ubiquitous expression, such as in rare allele lethal yellow, causes yellow coat., linear growth, immune defects and obesity. Agouti is a paracrine-signaling molecule that affects pigmentation by inhibiting the melanocortin receptor 1 (MCR-1 and possibly MC2R and MC4R).

Molecular Biology; MB-Proteins

ELISA (EL/EIA)

Suitable for use in Antibody Blocking, ELISA. Not suitable for use in Western Blot due to low molecular weight.
Dilution: Antibody Blockingt: 5-10ug per 1ug A1059-58 (anffinity purified antibody) or 1ul A1059-59B (antiserum).
ELISA: 50-100ng control peptide per well.
Optimal dilutions to be determined by researcher.

14,515 Da[Similar Products]

agouti signaling protein

agouti-signaling protein; nonagouti homolog; agouti switch protein; agouti signaling protein, nonagouti homolog

Agouti-signaling protein

AGTI; AGTIL; ASP; ASP  [Similar Products]

ASIP_HUMAN

In mice, the agouti gene encodes a paracrine signaling molecule that causes hair follicle melanocytes to synthesize pheomelanin, a yellow pigment, instead of the black or brown pigment, eumelanin. Pleiotropic effects of constitutive expression of the mouse gene include *****-onset obesity, increased tumor susceptibility, and premature infertility. This gene is highly similar to the mouse gene and encodes a secreted protein that may (1) affect the quality of hair pigmentation, (2) act as a pharmacological antagonist of alpha-melanocyte-stimulating hormone, (3) play a role in neuroendocrine aspects of melanocortin action, and (4) have a functional role in regulating lipid metabolism in adipocytes. [provided by RefSeq, Jul 2008]

ASIP: Involved in the regulation of melanogenesis. The binding of ASP to MC1R precludes alpha-MSH initiated signaling and thus blocks production of cAMP, leading to a down-regulation of eumelanogenesis (brown/black pigment) and thus increasing synthesis of pheomelanin (yellow/red pigment). In higher primates, agouti may affect the quality of hair pigmentation rather than its pattern of deposition. Could well play a role in neuroendocrine aspects of melanocortin action. May have some functional role in regulating the lipid metabolism with adipocytes.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q11.2-q12

Cellular Component: extracellular space

Molecular Function: type 3 melanocortin receptor binding; type 4 melanocortin receptor binding; receptor binding

Biological Process: melanin biosynthetic process; generation of precursor metabolites and energy; regulation of molecular function, epigenetic; hormone-mediated signaling; cell-cell signaling; melanosome organization and biogenesis; ***** feeding behavior; signal transduction; melanosome transport

Disease: Skin/hair/eye Pigmentation, Variation In, 9

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