AP-4 complex subunit sigma-1 (AP4S1), ELISA Kit

AP47B; CLA20; CLAPS4; FLJ32366; clathrin-associated/assembly/adaptor protein; sigma 4

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Mouse AP4S1. No significant cross-reactivity or interference between Mouse AP4S1 and analogues was observed.

Store at 2-8 degree C.

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Small volumes of AP4S1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2800238 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AP-4 complex subunit sigma-1 (AP4S1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AP4S1. The ELISA analytical biochemical technique of the MBS2800238 kit is based on AP4S1 antibody-AP4S1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AP4S1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AP4S1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

This assay employs a two-site sandwich ELISA to quantitate AP4S1 in samples. An antibody specific for AP4S1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AP4S1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AP4S1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AP4S1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

16,818 Da

adaptor-related protein complex AP-4, sigma 1

AP-4 complex subunit sigma-1

AP-4 complex subunit sigma-1

Sigma4-adaptin  [Similar Products]

This gene encodes the sigma subunit of the adaptor-related protein complex 4 which mediates intracellular membrane trafficking along the endocytic and secretory transport pathways. This complex contains four subunits, beta, epsilon, mu, and sigma, and belongs to a family of five adapter protein complexes, including three clathrin-associated complexes and two non clathrin-associated complexes, that localize to different intracellular compartments and mediate membrane vesicle trafficking using distinct pathways. In humans, loss-of-function mutations in this gene have been linked to specific adapter complex 4 deficiency disorders including hereditary spastic paraplegia. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016]

Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways. AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons. AP-4 is involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos, but may also recognize other types of sorting signal.

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