Product Name
Agouti-signaling protein (ASIP), ELISA Kit
Full Product Name
Cat Agouti-signaling protein (ASIP) ELISA Kit
Product Synonym Names
Agouti Signalling Peptide (ASIP)
Product Gene Name
ASIP elisa kit
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
3D Structure
ModBase 3D Structure for Q865F0
Specificity
This assay has high sensitivity and excellent specificity for detection of Cat ASIP. No significant cross-reactivity or interference between Cat ASIP and analogues was observed.
Samples
Serum, Plasma, Other
biological fluids
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ASIP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ASIP purchase
MBS284508 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Agouti-signaling protein (ASIP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ASIP. The ELISA analytical biochemical technique of the MBS284508 kit is based on ASIP antibody-ASIP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ASIP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ASIP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ASIP elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ASIP in samples. An antibody specific for ASIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyASIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ASIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ASIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for ASIP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001009190.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001009190.1
[Other Products]
UniProt Primary Accession #
Q865F0
[Other Products]
UniProt Related Accession #
Q865F0[Other Products]
Molecular Weight
14,948 Da
NCBI Official Full Name
agouti-signaling protein
NCBI Official Symbol
ASIP [Similar Products]
NCBI Protein Information
agouti-signaling protein
UniProt Protein Name
Agouti-signaling protein
UniProt Synonym Protein Names
Agouti switch protein
Protein Family
Agouti-signaling protein
UniProt Gene Name
ASIP [Similar Products]
UniProt Synonym Gene Names
ASP [Similar Products]
UniProt Comments for ASIP
Involved in the regulation of melanogenesis. The binding of ASP to MC1R precludes alpha-MSH initiated signaling and thus blocks production of cAMP, leading to a down-regulation of eumelanogenesis (brown/black pigment) and thus increasing synthesis of pheomelanin (yellow/red pigment) ().
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
