Matrix Gla Protein, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of MGP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS740932 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Matrix Gla Protein (MGP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MGP. The ELISA analytical biochemical technique of the MBS740932 kit is based on MGP antibody-MGP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MGP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MGP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: MGP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MGP antibody and an MGP-HRP conjugate. The assay sample and buffer are incubated together with MGP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MGP concentration since MGP from samples and MGP-HRP conjugate compete for the anti-MGP antibody . Since the number of sites is limited, as more sites are occupied by MGP from the sample, fewer sites are left to bind MGP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MGP concentration in each sample is interpolated from this standard curve.

Signal Transduction

15,325 Da

matrix Gla protein

matrix Gla protein; cell growth-inhibiting gene 36 protein

Matrix Gla protein

MGLAP; GIG36; MGP  [Similar Products]

MGP_HUMAN

The protein encoded by this gene is secreted and likely acts as an inhibitor of bone formation. The encoded protein is found in the organic matrix of bone and cartilage. Defects in this gene are a cause of Keutel syndrome (KS). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

MGP: Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation. Defects in MGP are the cause of Keutel syndrome (KS). KS is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia. Belongs to the osteocalcin/matrix Gla protein family.

Protein type: Secreted, signal peptide; Extracellular matrix; Secreted

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: protein binding; structural constituent of bone; extracellular matrix structural constituent; calcium ion binding

Biological Process: ossification; cell differentiation; regulation of bone mineralization; cartilage condensation

Disease: Keutel Syndrome

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