CAVIN1, Polyclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Affinity Purified

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and may contain up to 2% sucrose.

For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-CAVIN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This is a rabbit polyclonal antibody against PTRF. It was validated on Western Blot using a cell lysate as a positive control.

Polyclonal; Transcription Factor; Signal Proteins; Transcription Regulation; Chromatin & Nuclear Signaling; Disease Related; DNA/RNA/Protein Interactions; Immunohistochemistry; Transcription Factors;

Immunohistochemistry (IHC), Western Blot (WB)

IHC Information: Paraffin embedded mouse vessel and adipocytes (skeletal muscle) tissue, tested with an antibody Dilution of 5 ug/ml.

Host: Rabbit
Target Name: PTRF
Sample Tissue: Human Fetal Lung
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: PTRF
Sample Tissue: Mouse Lung
Antibody Dilution: 1.0ug/ml

Lanes:
Lane 1: 50ug mouse embryonic fibroblast lysate
Lane 2: 50ug mouse PTRF-KO embryonic fibroblast lysate
Lane 3: 50ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF protein
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Donkey anti-Rabbit-HRP
Secondary Antibody Dilution:
1:10000
Gene Name:
PTRF
Submitted by:
Libin Liu, Boston University.

WB Suggested Anti-PTRF Antibody Titration: 0.625ug/ml
ELISA Titer: 1:312500
Positive Control: NIH/3T3 cell lysate

43kDa

caveolae associated 1

caveolae-associated protein 1; polymerase I and transcript release factor

Polymerase I and transcript release factor

PTRF_MOUSE

PTRF: Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex. Defects in PTRF are the cause of congenital generalized lipodystrophy type 4 (CGL4). It is a disorder characterized by the association of congenital generalized lipodystrophy with muscular dystrophy and cardiac anomalies. Congenital generalized lipodystrophy is characterized by a near complete absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. Belongs to the PTRF/SDPR family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription initiation complex

Cellular Component: protein complex; membrane; intracellular membrane-bound organelle; mitochondrion; endoplasmic reticulum; cytoplasm; plasma membrane; caveola; nucleus

Molecular Function: rRNA binding; rRNA primary transcript binding; RNA binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter; transcription termination

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.