BRD3, Monoclonal Antibody

Homo sapiens bromodomain containing 3 (BRD3), mRNA; ORFX; RING3L; KIAA0043

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

2088C3a

Mouse

100 ug/ml (1.0 ml) (lot specific)

Small volumes of anti-BRD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known. [NCBI Entrez Gene Summary]

Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)

Western blot analysis of immunized recombinant protein, using anti-BRD3 monoclonal antibody.

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.

Detection of BRD3 by Western blot.

Samples: Whole cell lysate from human HeLa (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. 2088C3a-1]

Predicted molecular weight: 79 kDa

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-BRD3 mAb. [Lot No. 2088C3a-1]

Predicted molecular weight: 79 kDa

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-BRD3 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2088C3a-1]

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BRD3 mAb. [Lot No. 2088C3a-1]

60,942 Da

bromodomain containing 3

bromodomain-containing protein 3; RING3-like protein; bromodomain-containing 3

Bromodomain-containing protein 3

BRD3_HUMAN

This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known. [provided by RefSeq, Jul 2008]

BRD3: Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets. Regulates transcription of the CCND1 gene. A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, atypical; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; BRD family

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: nucleus

Molecular Function: protein binding; chromatin binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; chromatin modification

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