CD283, Monoclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a

11F8

Rat

FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

IgG concentration 0.1mg/ml (lot specific)

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Small volumes of anti-CD283 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rat anti Mouse CD283 anribody, clone 11F8 recognizes mouse TLR3, otherwise known as CD283 or Toll-like receptor 3. CD283 is a 905 amino acid 𞲙 kDa single pass type 1 transmembrane glycoprotein associated with endosomal membranes and member of the Toll-like receptor (TLR) family. CD283 functions as a receptor for double-stranded RNA (UniProt: Q99MB1).TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents.They also initiate cell signalling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity.

Flow cytometry (FC/FACS), Immunofluorescence (IF)

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

Mouse spleen cells stained withRat anti Mouse CD8 alpha:APC (MBS210027) and (A) Rat anti Mouse CD283 (MBS224437) or (B) rat IgG control. Detection with Goat anti Rat IgG:Dylight^®488 was performed following permeabilisation of the cells withLeucoperm^™ prior to incubation with Rat anti Mouse CD283 (MBS224437)

Mouse spleen cells stained withRat anti Mouse CD8 alpha:APC (MBS210027) and (A) Rat anti Mouse CD283:FITC (MBS224455) or (B) rat IgG control. Staining was performed following permeabilisation of the cells withLeucoperm^™ and incubation withMouse Seroblock FCR

103,671 Da

toll-like receptor 3

toll-like receptor 3

Toll-like receptor 3

TLR3_MOUSE

TLR3: a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. Most abundantly expressed in placenta and pancreas. Restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection. Acts via MyD88 and TRAF6, leading to the activation of NF-kappaB and the production of type I interferon.

Protein type: Membrane protein, integral; Receptor, cytokine

Cellular Component: cell surface; membrane; endoplasmic reticulum; lysosomal membrane; cytoplasm; integral to membrane; intracellular; endosome

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; RNA binding; double-stranded RNA binding; receptor activity

Biological Process: positive regulation of interleukin-12 production; positive regulation of JNK cascade; microglial cell activation; positive regulation of interferon-alpha biosynthetic process; defense response; positive regulation of NF-kappaB import into nucleus; toll-like receptor 3 signaling pathway; signal transduction; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to exogenous dsRNA; positive regulation of interferon-beta production; positive regulation of interferon type I production; inflammatory response; defense response to virus; positive regulation of cytokine production; positive regulation of I-kappaB kinase/NF-kappaB cascade; immune system process; MyD88-independent toll-like receptor signaling pathway; response to virus; response to dsRNA; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of toll-like receptor signaling pathway; positive regulation of chemokine biosynthetic process; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; I-kappaB phosphorylation; positive regulation of interferon-gamma biosynthetic process; microglial cell activation during immune response; positive regulation of inflammatory response

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