Full Product Name
Rabbit Anti Human TLR3 PolyClonal Antibody
Product Synonym Names
TLR3; CD283
Product Gene Name
anti-TLR3 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Immunogen
Recombinant protein of human TLR3
Relevance
Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses. TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-kappaB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IkappaB that normally maintains NF-kappaB inactivity by sequestering it in the cytoplasm.TLR3 functions as a receptor for double-stranded (ds)RNA typically associated with viral infection. It was originally shown to be specifically expressed in dendritic cells of the leukocyte family. TLR3 has also been found in placenta and lung, and can be induced by LPS in a variety of tissues. TLR3 is predominantly localized to early endosomes. Binding of dsRNA, or the analog polyinosine-polycytidylic acid (pIpC), to TLR3 triggers activation of transcription factors NF-kappaB and IRF3 through the adaptor protein TICAM-1/TRIF. TRIF associates with members of the TRAF family and with RIP that combine to activate NF-kappaB and IRF3.
Storage Buffer
Store at -20 degree C or -80 degree C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-TLR3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-TLR3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
NCBI/Uniprot data below describe general gene information for TLR3. It may not necessarily be applicable to this product.
NCBI Accession #
ABC86910.1
[Other Products]
UniProt Secondary Accession #
Q4VAL2; Q504W0; B2RAI7; B7Z7K0; E6Y0F0; E6Y0F1; E9PGH4[Other Products]
UniProt Related Accession #
O15455[Other Products]
Molecular Weight
72,937 Da
NCBI Official Full Name
TLR3
NCBI Official Synonym Full Names
toll-like receptor 3
NCBI Official Symbol
TLR3 [Similar Products]
NCBI Official Synonym Symbols
CD283; IIAE2
[Similar Products]
NCBI Protein Information
toll-like receptor 3
UniProt Protein Name
Toll-like receptor 3
UniProt Synonym Protein Names
CD_antigen: CD283
Protein Family
Toll-like receptor
UniProt Gene Name
TLR3 [Similar Products]
UniProt Entry Name
TLR3_HUMAN
NCBI Summary for TLR3
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for TLR3
TLR3: a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. Most abundantly expressed in placenta and pancreas. Restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection. Acts via MyD88 and TRAF6, leading to the activation of NF-kappaB and the production of type I interferon.
Protein type: Receptor, cytokine; Membrane protein, integral
Chromosomal Location of Human Ortholog: 4q35
Cellular Component: Golgi membrane; endoplasmic reticulum membrane; cell surface; membrane; integral to plasma membrane; lysosomal membrane; cytoplasm; endosome membrane
Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; double-stranded RNA binding; receptor activity
Biological Process: negative regulation of osteoclast differentiation; I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 production; positive regulation of apoptosis; positive regulation of JNK cascade; positive regulation of interferon-alpha biosynthetic process; positive regulation of NF-kappaB import into nucleus; toll-like receptor 3 signaling pathway; signal transduction; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to exogenous dsRNA; positive regulation of interferon-beta production; inflammatory response; defense response to virus; toll-like receptor 4 signaling pathway; MyD88-independent toll-like receptor signaling pathway; male gonad development; activation of NF-kappaB-inducing kinase; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of toll-like receptor signaling pathway; positive regulation of chemokine production; hyperosmotic response; positive regulation of chemokine biosynthetic process; detection of virus; positive regulation of interferon-beta biosynthetic process; defense response to bacterium; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; I-kappaB phosphorylation; microglial cell activation during immune response; positive regulation of interferon-gamma biosynthetic process; positive regulation of inflammatory response
Disease: Human Immunodeficiency Virus Type 1, Susceptibility To
Research Articles on TLR3
1. RNase L activity limits FFA/obesity-induced impairment of insulin response in muscle cells via TLR3 and MnSOD expression.
Precautions
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Disclaimer
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