Full Product Name
TLR3 siRNA (Human)
Product Synonym Names
Toll-like receptor 3; CD antigen CD283
Product Gene Name
TLR3 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O15455
Specificity
TLR3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TLR3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TLR3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TLR3 sirna
siRNA to inhibit TLR3 expression using RNA interference
Applications Tested/Suitable for TLR3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TLR3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_003256.1
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NCBI GenBank Nucleotide #
NM_003265.2
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UniProt Primary Accession #
O15455
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UniProt Secondary Accession #
Q4VAL2; Q504W0; B2RAI7; B7Z7K0; E6Y0F0; E6Y0F1; E9PGH4[Other Products]
UniProt Related Accession #
O15455[Other Products]
Molecular Weight
72,937 Da
NCBI Official Full Name
toll-like receptor 3
NCBI Official Synonym Full Names
toll-like receptor 3
NCBI Official Symbol
TLR3 [Similar Products]
NCBI Official Synonym Symbols
CD283; IIAE2
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NCBI Protein Information
toll-like receptor 3
UniProt Protein Name
Toll-like receptor 3
UniProt Synonym Protein Names
CD_antigen: CD283
Protein Family
Toll-like receptor
UniProt Gene Name
TLR3 [Similar Products]
UniProt Entry Name
TLR3_HUMAN
NCBI Summary for TLR3
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for TLR3
TLR3: a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. Most abundantly expressed in placenta and pancreas. Restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection. Acts via MyD88 and TRAF6, leading to the activation of NF-kappaB and the production of type I interferon.
Protein type: Receptor, cytokine; Membrane protein, integral
Chromosomal Location of Human Ortholog: 4q35
Cellular Component: Golgi membrane; endoplasmic reticulum membrane; cell surface; membrane; lysosomal membrane; integral to plasma membrane; cytoplasm; endosome membrane
Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; double-stranded RNA binding; receptor activity
Biological Process: negative regulation of osteoclast differentiation; I-kappaB kinase/NF-kappaB cascade; positive regulation of apoptosis; positive regulation of interleukin-12 production; positive regulation of JNK cascade; positive regulation of NF-kappaB import into nucleus; positive regulation of interferon-alpha biosynthetic process; toll-like receptor 3 signaling pathway; signal transduction; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to exogenous dsRNA; positive regulation of interferon-beta production; inflammatory response; toll-like receptor 4 signaling pathway; defense response to virus; MyD88-independent toll-like receptor signaling pathway; male gonad development; activation of NF-kappaB-inducing kinase; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of toll-like receptor signaling pathway; hyperosmotic response; positive regulation of chemokine biosynthetic process; detection of virus; positive regulation of interferon-beta biosynthetic process; defense response to bacterium; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; I-kappaB phosphorylation; positive regulation of interferon-gamma biosynthetic process; positive regulation of inflammatory response; microglial cell activation during immune response
Disease: Human Immunodeficiency Virus Type 1, Susceptibility To
Research Articles on TLR3
1. Increased risk of influenza for people with Leu412capital ER, Cyrilliche TLR-3 genotype
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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