DHCR24, Polyclonal Antibody

Delta(24)-sterol reductase; 24-dehydrocholesterol reductase; 3-beta-hydroxysterol delta-24-reductase; Diminuto/dwarf1 homolog; Seladin-1; DHCR24; KIAA0018

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This DHCR24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-87 amino acids from the N-terminal region of human DHCR24.

Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Vial Concentration: 2 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-DHCR24 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

DHCR24 is a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. This protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of its gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.

Cancer; Cardiovascular; Cell Biology; Metabolism; Neuroscience

Western Blot (WB), ELISA (EIA)

WB~~1:1000

Western blot analysis of anti-DHCR24 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). DHCR24 (arrow) was detected using the purified Pab.

Western blot analysis of anti-DHCR24 Antibody (N-term) in mouse liver tissue lysates (35ug/lane). DHCR24 (arrow) was detected using the purified Pab.

60101

24-dehydrocholesterol reductase

delta(24)-sterol reductase

Delta(24)-sterol reductase

KIAA0018  [Similar Products]

DHC24_HUMAN

This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells. [provided by RefSeq, Jul 2008]

Diminuto: the enzyme 3-beta-hydroxysterol delta-24-reductase (DHCR24), a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood cells. Overexpressed in the adenoma tissue of patients with Cushing's syndrome. May be involved in the molecular events of adrenocortical tumorigenesis by facilitating steroid synthesis and cell growth.

Protein type: EC 1.3.1.72; Oxidoreductase; Lipid Metabolism - steroid biosynthesis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; cytoskeleton; membrane; endoplasmic reticulum; integral to membrane; cytosol; nucleus

Molecular Function: delta24(24-1) sterol reductase activity; UDP-N-acetylmuramate dehydrogenase activity; enzyme binding; FAD binding; peptide antigen binding; oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; delta24-sterol reductase activity

Biological Process: skin development; tissue development; apoptosis; response to hormone stimulus; negative regulation of caspase activity; cholesterol biosynthetic process; negative regulation of cell proliferation; protein localization; amyloid precursor protein catabolic process; Ras protein signal transduction; male genitalia development; response to oxidative stress; cell cycle arrest; plasminogen activation; negative regulation of apoptosis

Disease: Desmosterolosis

Wang,Y., Mol. Pharmacol. 74 (6), 1716-1721 (2008)
Bonaccorsi,L., Lab. Invest. 88 (10), 1049-1056 (2008)
Cecchi,C., J. Cell. Mol. Med. 12 (5B), 1990-2002 (2008)

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