Product Name
Alpha-Hemoglobin (aHSP), Polyclonal Antibody
Popular Item
Full Product Name
Polyclonal Antibody to Alpha-Hemoglobin Stabilizing Protein (aHSP)
Product Gene Name
anti-aHSP antibody
[Similar Products]
Matching Pairs
Unconjugated Antibody: Alpha-Hemoglobin (MBS2028480)
Immunogen: Alpha-Hemoglobin (MBS2030839)
Matching Pairs
Unconjugated Antibody: Alpha-Hemoglobin (MBS2028480)
APC-CY7 Conjugated Antibody: Alpha-Hemoglobin Stabilizing Protein (aHSP) (MBS2042074)
Matching Pairs
Unconjugated Antibody: Alpha-Hemoglobin (MBS2028480)
PE Conjugated Antibody: Alpha-Hemoglobin Stabilizing Protein (aHSP) (MBS2042076)
Matching Pairs
Unconjugated Antibody: Alpha-Hemoglobin (MBS2028480)
APC Conjugated Antibody: Alpha-Hemoglobin Stabilizing Protein (aHSP) (MBS2042078)
Matching Pairs
Unconjugated Antibody: Alpha-Hemoglobin (MBS2028480)
Cy3 Conjugated Antibody: Alpha-Hemoglobin Stabilizing Protein (aHSP) (MBS2042080)
Matching Pairs
Unconjugated Antibody: Alpha-Hemoglobin (MBS2028480)
FITC Conjugated Antibody: Alpha-Hemoglobin Stabilizing Protein (aHSP) (MBS2042082)
Matching Pairs
Unconjugated Antibody: Alpha-Hemoglobin (MBS2028480)
HRP Conjugated Antibody: Alpha-Hemoglobin Stabilizing Protein (aHSP) (MBS2042084)
Matching Pairs
Unconjugated Antibody: Alpha-Hemoglobin (MBS2028480)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
Matching Pairs
Unconjugated Antibody: Alpha-Hemoglobin (MBS2028480)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
Matching Pairs
Unconjugated Antibody: Alpha-Hemoglobin (MBS2028480)
Biotin Conjugated Antibody: Alpha-Hemoglobin Stabilizing Protein (aHSP) (MBS2091969)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- APFQSNKDL ISTGIKEFNV LLDQQVFDDP LISEEDMVIV VHDWVNLYTN YYKKLVHGEQ EEQDRAMTEF QQELSTLGSQ FLAKYRTFLK SKEPPSNTLP SS
3D Structure
ModBase 3D Structure for Q9CY02
Specificity
The antibody is a rabbit polyclonal antibody raised against aHSP. It has beenselected for its ability to recognize aHSP in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
200ug/ml (lot specific)
Fragment
aHSP (Ala2~Ser102)
Organism Species
Mus musculus (Mouse)
Immunogen
Recombinant aHSP (Ala2~Ser102) with two N-terminal Tags, His-tag and T7-tag expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2042078
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customerâs specifications, please inquire.
Other Notes
Small volumes of anti-aHSP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-aHSP antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-aHSP antibody
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB) of anti-aHSP antibody
Western Blot: Sample: Recombinant protein.

Immunohistochemistry (IHC) of anti-aHSP antibody
DAB staining on fromalin fixed paraffin- embedded stomach tissue)

Immunohistochemistry (IHC) of anti-aHSP antibody
DAB staining on IHC-P; Samples: Mouse Stomach Tissue)

NCBI/Uniprot data below describe general gene information for aHSP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_573508.1
[Other Products]
NCBI GenBank Nucleotide #
NM_133245.1
[Other Products]
UniProt Primary Accession #
Q9CY02
[Other Products]
UniProt Secondary Accession #
O70427[Other Products]
UniProt Related Accession #
Q9CY02[Other Products]
Molecular Weight
11,832 Da
NCBI Official Full Name
alpha-hemoglobin-stabilizing protein
NCBI Official Synonym Full Names
alpha hemoglobin stabilizing protein
NCBI Official Symbol
Ahsp [Similar Products]
NCBI Official Synonym Symbols
EDRF; Eraf
[Similar Products]
NCBI Protein Information
alpha-hemoglobin-stabilizing protein
UniProt Protein Name
Alpha-hemoglobin-stabilizing protein
UniProt Synonym Protein Names
Erythroid differentiation-related factor; Erythroid-associated factor
Protein Family
Alpha-hemoglobin-stabilizing protein
UniProt Gene Name
Ahsp [Similar Products]
UniProt Synonym Gene Names
Edrf; Eraf [Similar Products]
UniProt Comments for aHSP
Eraf: Acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. Belongs to the AHSP family.
Protein type: Cell development/differentiation
Chromosomal Location of Human Ortholog: -
Cellular Component: cytoplasm
Molecular Function: hemoglobin binding
Biological Process: erythrocyte differentiation; protein folding; protein stabilization
Research Articles on aHSP
1. role of alpha-hemoglobin stabilizing protein in hemoglobin assembly
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.