c-Myb Oncoprotein, Monoclonal Antibody

c-myb_CDS; efg; ONCOGENE AMV; Oncogene MYB; Proto-oncogene c-Myb; Transcriptional activator Myb; v-myb avian myeloblastosis viral oncogene homolog

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1, kappa

MYB286

Mouse

The highly leukemogenic avian retrovirus E26 contains two oncogenes, v-Myb and v-Ets, which are expressed together as a fusion protein. The cellular hom- olog of v-Myb, designated c-Myb, encodes a transcription factor. Deletion or disruption of a negative regulatory domain mapping within the carboxy-ter- minal domain of c-Myb results in enhanced transactivating capacity and in parallel, leads to activation of its ability to transform hemopoietic cells. c-Myb is expressed preferentially, but not exclusively, in immature hemopoietic cells and its expression decreases as cells differentiate.

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Store at 2 to 8 degree C. Antibody is stable for 24 months.

Small volumes of anti-MYB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Immunohistology (IH)

ELISA (For coating, order Ab without BSA); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Western Blotting (0.5-1ug/ml); Immunoprecipitation (1-2ug/500ug protein lysate); Optimal dilution for a specific application should be determined.

~75kDa

v-myb avian myeloblastosis viral oncogene homolog

transcriptional activator Myb; c-myb8B_CDS; c-myb10A_CDS; c-myb13A_CDS; c-myb14A_CDS; proto-oncogene c-Myb; c-myb protein (140 AA)

Transcriptional activator Myb

MYB_HUMAN

This gene encodes a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]

Myb: Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6q22-q23

Cellular Component: nuclear matrix; cytosol

Molecular Function: protein binding; chromatin binding

Biological Process: positive regulation of histone H3-K9 methylation; thymus development; transcription, DNA-dependent; in utero embryonic development; positive regulation of transcription, DNA-dependent; homeostasis of number of cells; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; chromatin remodeling; positive regulation of T-helper cell differentiation; regulation of transcription, DNA-dependent; calcium ion transport; B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; blood coagulation; embryonic gut development; G1/S transition of mitotic cell cycle

1. Gonda, T.J., et al. 1984. Expression of Myb, Myc and Fos proto-oncogenes during the differentiation of a murine myeloid leukaemia. Nature 310: 249-251.

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