Product Name
Alpha-hemoglobin-stabilizing protein (ERAF), ELISA Kit
Full Product Name
Goat Alpha-hemoglobin-stabilizing protein (ERAF) ELISA Kit
Product Gene Name
ERAF elisa kit
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ERAF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ERAF purchase
MBS7225467 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alpha-hemoglobin-stabilizing protein (ERAF) ELISA Kit target analytes in
biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ERAF. The ELISA analytical biochemical technique of the MBS7225467 kit is based on ERAF antibody-ERAF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ERAF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ERAF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for ERAF elisa kit
Signal Transduction
NCBI/Uniprot data below describe general gene information for ERAF. It may not necessarily be applicable to this product.
NCBI Accession #
NP_057717.1
[Other Products]
NCBI GenBank Nucleotide #
NM_016633.2
[Other Products]
UniProt Secondary Accession #
Q8TD01[Other Products]
UniProt Related Accession #
Q9NZD4[Other Products]
Molecular Weight
11,840 Da
NCBI Official Full Name
alpha-hemoglobin-stabilizing protein
NCBI Official Synonym Full Names
alpha hemoglobin stabilizing protein
NCBI Official Symbol
AHSP [Similar Products]
NCBI Official Synonym Symbols
EDRF; ERAF
[Similar Products]
NCBI Protein Information
alpha-hemoglobin-stabilizing protein; erythroid associated factor; erythroid-associated factor; alpha hemoglobin stabilising protein; erythroid differentiation-related factor; erythroid differentiation associated factor
UniProt Protein Name
Alpha-hemoglobin-stabilizing protein
UniProt Synonym Protein Names
Erythroid differentiation-related factor; Erythroid-associated factor
UniProt Gene Name
AHSP [Similar Products]
UniProt Synonym Gene Names
EDRF; ERAF [Similar Products]
UniProt Entry Name
AHSP_HUMAN
UniProt Comments for ERAF
AHSP: Acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia. Belongs to the AHSP family.
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: hemoglobin complex
Molecular Function: hemoglobin binding; unfolded protein binding
Biological Process: protein stabilization; protein folding; hemoglobin metabolic process; hemopoiesis
Research Articles on ERAF
1. alpha-Hemoglobin-stabilizing protein (AHSP) perturbs the proximal heme pocket of oxy-alpha-hemoglobin and weakens the iron-oxygen bond.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.