MeCP2, Peptide

For Research Use Only. Not for use in diagnostic procedures.

50 ug (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.
Appearance: Colorless liquid

0.5 mg/ml (lot specific)

At -20 degree C
Shelf Life: 12 months

Small volumes of MeCP2 peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Epigenetics; Writer Enzymes; DNA Methyltransferases (DNMTs); Antibodies and Blocking PeptidesAntibodies & Supporting Tools; Blocking Peptides

Western Blot (WB)

The peptide is used for blocking the antibody MeCP2. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37 degree C.

53,323 Da

methyl CpG binding protein 2

methyl-CpG-binding protein 2

Methyl-CpG-binding protein 2

MeCp-2 protein; MeCp2  [Similar Products]

MECP2_HUMAN

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq, Jul 2009]

MECP2: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Interacts with FNBP3. Interacts with CDKL5. Present in all ***** somatic tissues tested. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: nucleoplasm; heterochromatin; extracellular space; nucleus; cytosol

Molecular Function: mRNA binding; protein domain specific binding; protein binding; siRNA binding; methyl-CpG binding; DNA binding; double-stranded methylated DNA binding; protein N-terminus binding; chromatin binding; transcription factor activity; transcription corepressor activity; transcription factor binding

Biological Process: catecholamine secretion; startle response; genetic imprinting; behavioral fear response; ***** locomotory behavior; positive regulation of transcription, DNA-dependent; chromatin silencing; pathogenesis; neurological control of breathing; proprioception; negative regulation of transcription from RNA polymerase II promoter; sensory perception of pain; negative regulation of histone methylation; neuron maturation; negative regulation of histone acetylation; post-embryonic development; phosphatidylcholine metabolic process; protein localization; synaptogenesis; positive regulation of cell proliferation; dendrite development; cerebellum development; visual learning; negative regulation of neuron apoptosis; histone acetylation; mitochondrial electron transport, ubiquinol to cytochrome c; transcription, DNA-dependent; ventricular system development; glutamine metabolic process; respiratory gaseous exchange; social behavior; cardiolipin metabolic process; histone methylation; positive regulation of synaptogenesis; glucocorticoid metabolic process; regulation of systemic arterial blood pressure by neurological process; long-term memory; inositol metabolic process; response to hypoxia; negative regulation of transcription, DNA-dependent; regulation of excitatory postsynaptic membrane potential

Disease: Rett Syndrome; Mental Retardation, X-linked, Syndromic 13; Autism, Susceptibility To, X-linked 3; Lubs X-linked Mental Retardation Syndrome; Encephalopathy, Neonatal Severe, Due To Mecp2 Mutations; Angelman Syndrome

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.