DNM1L / DRP1, Polyclonal Antibody

Anti-DNM1L / DRP1 Antibody (Internal) IHC-plus; DNM1L; DYMPLE; Dynamin 1-like; Dynamin-like protein; Dynamin-like protein 4; Dynamin-like protein IV; DLP1; Dnm1p/Vps1p-like protein; DVLP; Dynamin-related protein 1; DYNIV-11; DRP1; Dynamin-1-like protein; VPS1; EMPF; HDYNIV; Human DNM1L; DRP1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

DNM1L antibody is human and mouse reactive. At least four isoforms of DNM1L are known to exist; this antibody will detect the two longest isoforms.

Immunoaffinity Purified

PBS, 0.02% sodium azide.

1 mg/ml (lot specific)

Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Small volumes of anti-DNM1L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

DNM1L/DRP1 is a GTPase with a domain structure similar to that of other dynamin family members. Studies with mutated DNM1L have demonstrated its role in distributing mitochondrial tubules throughout the cytoplasm. It has also been shown to induce mitochondrial fragmentation and programmed cell death during C. elegans development.

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)

ELISA, ICC (5 ug/ml), IF (20 ug/ml), IHC-P (10 ug/ml), WB (1 - 2 ug/ml)

Anti-DNM1L / DRP1 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunofluorescence of DNM1L in HeLa cells with DNM1L antibody at 20 µg/mL.

Immunocytochemistry of DNM1L in HeLa cells with DNM1L antibody at 5 µg/mL.

Western blot analysis of DNM1L in HeLa cell lysate with DNM1L antibody at 1 ug/ml.

82,057 Da

dynamin 1-like

dynamin-1-like protein; dynamin-like protein 4; dynamin-like protein IV; Dnm1p/Vps1p-like protein; dynamin-related protein 1; dynamin family member proline-rich carboxyl-terminal domain less

Dynamin-1-like protein

DLP1; DRP1; DVLP; Dymple; HdynIV  [Similar Products]

DNM1L_HUMAN

This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]

DRP1: Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis- dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport. Homotetramer; dimerizes through the N-terminal GTP-middle region of one molecule binding to the GED domain of another DNM1L molecule. Can self-assemble in multimeric ring-like structures. Interacts with BCL2L1; the interaction stimulates the GTPase activity of DMN1L in synapses and increases the number of axonal mitochondria and the size and number of synaptic vesicle clusters. Interacts with FIS1. Interacts with GSK3B and MARCH5. Interacts (via the GTPase and B domains) with UBE2I; the interaction promotes sumoylation of DNM1L, mainly in ite B domain. Interacts with PPP3CA; the interaction dephosphorylates DNM1L and regulates its transition to mitochondria. Interacts witn MID49 and MID51. Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons. Belongs to the dynamin family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Microtubule-binding; Mitochondrial; Motor; Hydrolase; EC 3.6.5.5; Apoptosis; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 12p11.21

Cellular Component: Golgi apparatus; microtubule; protein complex; mitochondrion; intracellular membrane-bound organelle; synaptic vesicle membrane; coated pit; cytosol; mitochondrial outer membrane; membrane; perinuclear region of cytoplasm; cytoplasm; peroxisome; cell junction

Molecular Function: GTPase activity; identical protein binding; protein binding; protein homodimerization activity; GTP binding; ubiquitin protein ligase binding; lipid binding

Biological Process: peroxisome fission; mitochondrial fission; release of cytochrome c from mitochondria; positive regulation of protein secretion; metabolic process; apoptosis; positive regulation of apoptosis; regulation of protein oligomerization; endocytosis; mitochondrial fragmentation during apoptosis; protein homotetramerization; cell structure disassembly during apoptosis

Disease: Encephalopathy, Lethal, Due To Defective Mitochondrial And Peroxisomal Fission

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