CCM2, Recombinant Protein

Malcavernin isoform 4; Malcavernin isoform 4; Malcavernin isoform 4

For Research Use Only. Not for use in diagnostic procedures.

E Coli

> 90% by SDS-PAGE

Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 1mM DTT

0.5 mg/ml (determined by Bradford assay) (lot specific)

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of CCM2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

CCM2 provides instructions for making a protein called malcavernin, which strengthens the interactions between cells that form blood vessels and limits leakage from the vessels. It interacts with a number of other proteins to form a complex that is found in the junctions that connect neighboring cells. It helps turn off (suppress) a signaling molecule known as RhoA-GTPase. Recombinant human CCM2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Signal Transduction

SDS-PAGE

34.3kDa (311aa) confirmed by MALDI-TOF

CCM2 scaffolding protein

cerebral cavernous malformations 2 protein

Cerebral cavernous malformations 2 protein

C7orf22  [Similar Products]

This gene encodes a scaffold protein that functions in the stress-activated p38 Mitogen-activated protein kinase (MAPK) signaling cascade. The protein interacts with SMAD specific E3 ubiquitin protein ligase 1 (also known as SMURF1) via a phosphotyrosine binding domain to promote RhoA degradation. The protein is required for normal cytoskeletal structure, cell-cell interactions, and lumen formation in endothelial cells. Mutations in this gene result in cerebral cavernous malformations. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2009]

Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. May act through the stabilization of endothelial cell junctions (). May function as a scaffold protein for MAP2K3-MAP3K3 signaling. Seems to play a major role in the modulation of MAP3K3-dependent p38 activation induced by hyperosmotic shock ().

Dupre N. et al. (2003) Can J Neurol Sci. 30:122-128; Liquori CL. et al. (2007) Am J Hum Genet. 80:69-75.;

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