Neuroblastoma RAS Viral Oncogene Homolog, Recombinant Protein

GTPase NRas, HRAS1, ALPS4, N-ras, NRAS1, NS6, Transforming protein N-Ras.

For Research Use Only. Not for use in diagnostic procedures.

E Coli

Greater than 95.0% as determined by SDS-PAGE.

The NRAS protein solution (1mg/ml) contains 20mM Tris-HCl pH-7.5, 100mM NaCl, and 10% glycerol.

1mg/ml (lot specific)

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles

Small volumes of NRAS recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: NRAS Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 186 amino acids (1-186 a.a.) and having a molecular mass of 20.8kDa. The NRAS is purified by conventional chromatography.

Introduction: NRAS binds GDP/GTP and has intrinsic GTPase activity. NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This transport is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. NRAS is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this NRAS gene result in juvenile myelomonocytic leukemia.

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins

neuroblastoma RAS viral (v-ras) oncogene homolog

GTPase NRas; N-ras protein part 4; transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog

GTPase NRas

HRAS1  [Similar Products]

RASN_HUMAN

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]

NRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein; G protein, monomeric; G protein, monomeric, Ras; Oncoprotein; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: Golgi membrane; membrane; plasma membrane

Molecular Function: GTP binding; protein complex binding

Biological Process: regulation of long-term neuronal synaptic plasticity; epidermal growth factor receptor signaling pathway; negative regulation of skeletal muscle development; axon guidance; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; activation of MAPKK activity; MAPKKK cascade; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; small GTPase mediated signal transduction; positive regulation of cell proliferation; Ras protein signal transduction; insulin receptor signaling pathway; innate immune response; striated muscle cell differentiation; visual learning; negative regulation of neuron apoptosis; blood coagulation; actin cytoskeleton organization and biogenesis; vascular endothelial growth factor receptor signaling pathway; leukocyte migration

Disease: Thyroid Carcinoma, Follicular; Ras-associated Autoimmune Leukoproliferative Disorder; Schimmelpenning-feuerstein-mims Syndrome; Noonan Syndrome 6; Melanocytic Nevus Syndrome, Congenital; Colorectal Cancer; Melanosis, Neurocutaneous; Nevus, Epidermal

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