Rab27a, Polyclonal Antibody

Anti-Rab27a; GS2; GTP-binding protein Ram; rab-27; ras-related protein Rab-27A; RAB27; RAM

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 15; NC_000015.10 (55202966..55289832, complement). Location: 15q15-q21.1

Polyclonal

IgG

Goat

Detects Rab27a by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab27a.

Polyclonal antibody supplied as (1 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.

Small volumes of anti-Rab27a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

RAB27A, member RAS oncogene family

Goat polyclonal antibody to mouse Rab27a. Rab27a belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and involved in lysosome-related vesicle transport and small GTPase mediated signal transduction.

Immunofluorescence (IF), Western Blot (WB)

Western Blot: 1:250-1:1,000
Immunofluorescence: 1:50-1:200

24,868 Da

RAB27A, member RAS oncogene family

ras-related protein Rab-27A; rab-27; GTP-binding protein Ram

Ras-related protein Rab-27A

RAB27; Rab-27  [Similar Products]

RB27A_HUMAN

The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Function: Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Ref.15

Subunit structure: Binds SYTL1, SLAC2B, MYRIP, SYTL3, SYTL4 and SYTL5. Interacts with RPH3A and RPH3A

By similarity. Binds MLPH and SYTL2. Interacts with UNC13D. Ref.10 Ref.12 Ref.14 Ref.15

Subcellular location: Membrane; Lipid-anchor. Melanosome. Late endosome. Lysosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes to endosomal exocytic vesicles. Ref.11 Ref.12 Ref.13 Ref.14

Tissue specificity: Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells. Ref.12

Involvement in disease: Griscelli syndrome 2 (GS2) [MIM:607624]: Rare autosomal recessive disorder that results in pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes. GS2 patients also develop an uncontrolled T-lymphocyte and macrophage activation syndrome, known as hemophagocytic syndrome, leading to death in the absence of bone marrow transplantation. Neurological impairment is present in some patients, likely as a result of hemophagocytic syndrome.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.12 Ref.17 Ref.20 Ref.21

Sequence similarities: Belongs to the small GTPase superfamily. Rab family.

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