Product Name
PITX1, siRNA
Full Product Name
PITX1 siRNA (Mouse)
Product Synonym Names
BFT; POTX; PTX1; Pituitary homeobox 1; Hindlimb-expressed homeobox protein backfoot; Homeobox protein P-OTX; Homeobox protein PITX1; Paired-like homeodomain transcription factor 1; Pituitary OTX-related factor
Product Gene Name
PITX1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P70314
Specificity
PITX1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PITX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PITX1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PITX1 sirna
siRNA to inhibit PITX1 expression using RNA interference
Applications Tested/Suitable for PITX1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PITX1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_035227.1
[Other Products]
NCBI GenBank Nucleotide #
NM_011097.2
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UniProt Primary Accession #
P70314
[Other Products]
UniProt Secondary Accession #
O54742; P70427[Other Products]
UniProt Related Accession #
P70314[Other Products]
Molecular Weight
34,075 Da
NCBI Official Full Name
pituitary homeobox 1
NCBI Official Synonym Full Names
paired-like homeodomain transcription factor 1
NCBI Official Symbol
Pitx1 [Similar Products]
NCBI Official Synonym Symbols
Bft; Potx; Ptx1; P-OTX
[Similar Products]
NCBI Protein Information
pituitary homeobox 1
UniProt Protein Name
Pituitary homeobox 1
UniProt Synonym Protein Names
Hindlimb-expressed homeobox protein backfoot; Homeobox protein P-OTX; Homeobox protein PITX1; Paired-like homeodomain transcription factor 1; Pituitary OTX-related factor
Protein Family
Pituitary homeobox
UniProt Gene Name
Pitx1 [Similar Products]
UniProt Synonym Gene Names
Bft; Potx; Ptx1 [Similar Products]
UniProt Entry Name
PITX1_MOUSE
UniProt Comments for PITX1
PITX1: May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb. Defects in PITX1 are a cause of congenital clubfoot (CCF); also known as talipes equinovarus (TEV). Clubfoot is a congenital limb deformity defined as fixation of the foot in cavus, adductus, varus, and equinus (i.e. inclined inwards, axially rotated outwards, and pointing downwards) with concomitant soft tissue abnormalities. Clubfoot may occur in isolation or as part of a syndrome. Clubfoot appears to be a multifactorial trait. Belongs to the paired homeobox family. Bicoid subfamily.
Protein type: DNA-binding
Cellular Component: transcription factor complex; cytoplasm; nucleolus; nucleus
Molecular Function: DNA binding; sequence-specific DNA binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; hindlimb morphogenesis; myoblast cell fate commitment; transcription, DNA-dependent; regulation of transcription, DNA-dependent; pituitary gland development; cartilage development; multicellular organismal development; branchiomeric skeletal muscle development; positive regulation of transcription from RNA polymerase II promoter; skeletal development; embryonic hindlimb morphogenesis
Research Articles on PITX1
1. Pitx1 influences hindlimb morphology both through the activation of hindlimb-specific enhancers as well as through the hindlimb-specific modulation of enhancers that are active in both sets of limbs
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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