CCKAR, Polyclonal Antibody

CCKRA; Cholecystokinin receptor type A; CCK-A receptor; CCK-AR; Cholecystokinin-1 receptor; CCK1-R

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Recognizes endogenous levels of CCKAR protein.

The antibody was purified by immunogen affinity chromatography.

Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

Shipped and store at 4 degree C for one year. Do not freeze.

Small volumes of anti-CCKAR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rabbit polyclonal antibody to CCKAR

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Western Blot: (1/500 - 1/1000); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500); Immunoprecipitation: (1/10 - 1/100)

Western blot analysis of CCKAR expression in HeLa (A), Jurkat (B), PC12 (C) whole cell lysates.

Immunofluorescent analysis of CCKAR staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

47,841 Da

cholecystokinin A receptor

cholecystokinin receptor type A

Cholecystokinin receptor type A

CCKRA; CCK-A receptor; CCK-AR; CCK1-R  [Similar Products]

CCKAR_HUMAN

This gene encodes a G-protein coupled receptor that binds non-sulfated members of the cholecystokinin (CCK) family of peptide hormones. This receptor is a major physiologic mediator of pancreatic enzyme secretion and smooth muscle contraction of the gallbladder and stomach. In the central and peripheral nervous system this receptor regulates satiety and the release of beta-endorphin and dopamine. [provided by RefSeq, Jul 2008]

CCKAR: Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: cytosol; endoplasmic reticulum; endosome; integral to plasma membrane; lysosome; plasma membrane; terminal button

Molecular Function: cholecystokinin receptor activity

Biological Process: actin cytoskeleton reorganization; axonogenesis; cellular response to hormone stimulus; digestion; elevation of cytosolic calcium ion concentration; feeding behavior; forebrain development; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); gastric acid secretion; insulin secretion; neuron migration; organ regeneration; pancreas development; pancreatic juice secretion; reduction of food intake in response to dietary excess; regulation of calcium ion transport; regulation of hormone secretion; regulation of potassium ion transport; response to glucocorticoid stimulus; response to heat; response to lipopolysaccharide; response to nutrient; response to radiation; response to starvation; thermoregulation

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