CSTB, Monoclonal Antibody

CystatinB; Cystatin B; CSTB; Stefin B; PME; CST6; CystatinB CHROW21; CPI B; CYTB; EPM1; Liver thiol proteinase inhibitor; STFB.

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2b, k

2F1

Mouse

By protein-G affinity chromatography

Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.

1mg/ml (lot specific)

Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of anti-CSTB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

CSTB, also known as Cystatin B is an anti-protease implicated in myoclonus epilepsy, a degenerative disease of the central nervous system. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein is able to from a dimer stabilized by noncovalent forces and is thought to play a role in protecting against the proteases leaking from lysosomes in cells. CSTB is located in the lysosomes and the cytoplasm, but also in the nucleus.

Neuroscience

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

WB: 1:1,000~ 2,000.
Recommended starting dilution is 1:1,000.

The Cell lysates of A549 (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CSTB (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CSTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : U-87MG cell lysate

ICC/IF analysis of CSTB in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CSTB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of CSTB in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CSTB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

11,140 Da

cystatin B

cystatin-B

Cystatin-B

CST6; STFB  [Similar Products]

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies. [provided by RefSeq, Jul 2016]

This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.

Turk V, et al., (1991) FEBS Lett. 285 (2):213-9; Jarvinen M, et al., (1988) Acta Histochem. 82 (1):5-18

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