Rho guanine nucleotide exchange factor 6 (ARHGEF6), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ARHGEF6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7233797 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Rho guanine nucleotide exchange factor 6 (ARHGEF6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ARHGEF6. The ELISA analytical biochemical technique of the MBS7233797 kit is based on ARHGEF6 antibody-ARHGEF6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ARHGEF6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ARHGEF6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Signal Transduction

70,791 Da

Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6

rho guanine nucleotide exchange factor 6; PAK-interacting exchange factor alpha; PAK-interacting exchange factor, alpha; Rac/Cdc42 guanine exchange factor (GEF) 6; rac/Cdc42 guanine nucleotide exchange factor 6

Rho guanine nucleotide exchange factor 6

COOL2; KIAA0006; PIXA  [Similar Products]

ARHG6_HUMAN

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It may form a complex with G proteins and stimulate Rho-dependent signals. This protein is activated by PI3-kinase. Mutations in this gene can cause X-chromosomal non-specific mental retardation. [provided by RefSeq, Jul 2008]

ARHGEF6: Acts as a RAC1 guanine nucleotide exchange factor (GEF). Interacts with PAK kinases through the SH3 domain. Interacts with GIT1. Component of cytoplasmic complexes, which also contain PXN, GIT1 and PAK1. Ubiquitous. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Rac/Rho; Motility/polarity/chemotaxis; GEFs

Chromosomal Location of Human Ortholog: Xq26.3

Cellular Component: lamellipodium; intercellular junction; intracellular; cytosol

Molecular Function: Rho guanyl-nucleotide exchange factor activity; protein binding; GTPase activator activity

Biological Process: lamellipodium biogenesis; regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; small GTPase mediated signal transduction; JNK cascade; positive regulation of GTPase activity

Disease: Mental Retardation, X-linked 46

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