DNA Mismatch Repair Protein MSH2 (MSH2), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of MSH2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9399285 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DNA Mismatch Repair Protein MSH2 (MSH2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MSH2. The ELISA analytical biochemical technique of the MBS9399285 kit is based on MSH2 antibody-MSH2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MSH2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MSH2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

97,323 Da

DNA mismatch repair protein Msh2

hMSH2  [Similar Products]

MSH2: Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2- MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis. Heterodimer consisting of MSH2-MSH6 (MutS alpha) or MSH2- MSH3 (MutS beta). Both heterodimer form a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Part of the BRCA1- associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ATR. Interacts with SLX4/BTBD12; this interaction is direct and links MutS beta to SLX4, a subunit of different structure-specific endonucleases. Interacts with SMARCAD1. Ubiquitously expressed. Belongs to the DNA mismatch repair MutS family. Two isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Tumor suppressor

Chromosomal Location of Human Ortholog: 2p21-p16.3

Cellular Component: membrane; MutSalpha complex; MutSbeta complex; nuclear chromosome, telomeric region; nucleoplasm

Molecular Function: ADP binding; ATP binding; ATPase activity; centromeric DNA binding; dinucleotide insertion or deletion binding; dinucleotide repeat insertion binding; DNA binding; DNA-dependent ATPase activity; double-strand/single-strand DNA junction binding; double-stranded DNA binding; enzyme binding; four-way junction DNA binding; guanine/thymine mispair binding; loop DNA binding; magnesium ion binding; mismatched DNA binding; MutLalpha complex binding; oxidized purine DNA binding; protein binding; protein C-terminus binding; protein homodimerization activity; protein kinase binding; single guanine insertion binding; single thymine insertion binding; single-stranded DNA binding; Y-form DNA binding

Biological Process: B cell differentiation; B cell mediated immunity; cell cycle arrest; determination of ***** lifespan; DNA repair; double-strand break repair; germ cell development; in utero embryonic development; intra-S DNA damage checkpoint; intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; isotype switching; maintenance of DNA repeat elements; male gonad development; meiotic gene conversion; mismatch repair; negative regulation of DNA recombination; negative regulation of neuron apoptosis; negative regulation of reciprocal meiotic recombination; oxidative phosphorylation; positive regulation of helicase activity; positive regulation of isotype switching to IgA isotypes; positive regulation of isotype switching to IgG isotypes; postreplication repair; response to UV-B; response to X-ray; somatic hypermutation of immunoglobulin genes; somatic recombination of immunoglobulin gene segments

Disease: Lynch Syndrome I; Mismatch Repair Cancer Syndrome; Muir-torre Syndrome

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