SMARCAL1, Polyclonal Antibody

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1; HepA-related protein; hHARP; Sucrose nonfermenting protein 2-like 1; SMARCAL1; HARP

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

>95%, Protein G purified

Liquid

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-SMARCAL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks.

ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)

WB: 1:500-1:5000
IF: 1:50-1:200

Western Blot
Positive WB detected in: Jurkat whole cell lysate,Mouse lung tissue,Mouse kidney tissue
All lanes: SMARCAL1 antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 106 KDa
Observed band size: 106 KDa

Immunofluorescent analysis of Hela cells using MBS7053325 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

105,938 Da

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a like 1

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1

HARP; hHARP  [Similar Products]

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency. [provided by RefSeq, Jul 2008]

SMARCAL1: ATP-dependent annealing helicase that catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. Defects in SMARCAL1 are a cause of Schimke immuno-osseous dysplasia (SIOD). SIOD causes spondyloepiphyseal dysplasia, renal dysfunction and T-cell immunodeficiency. Approximately half of all patients also exhibit hyperthyroidism, while around half also exhibit episodal cerebral ischema. Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.

Protein type: EC 3.6.1.-; EC 3.6.4.-; Helicase

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: DNA replication factor A complex; nucleoplasm; nucleus

Molecular Function: DNA-dependent ATPase activity; protein binding

Biological Process: DNA metabolic process; regulation of transcription from RNA polymerase II promoter; replication fork processing; response to DNA damage stimulus

Disease: Immunoosseous Dysplasia, Schimke Type

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