Full Product Name
ATG9B Antibody: Alkaline Phosphatase
Product Synonym Names
Rabbit Anti-Human ATG9B Polyclonal; APG9-like 2 Antibody; APG9L2 Antibody; Nitric oxide synthase 3-overlapping antisense gene protein Antibody; Protein sONE Antibody; NOS3AS Antibody; ATG9B Antibody; Autophagy-related protein 9B Antibody
Product Gene Name
anti-ATG9B antibody
[Similar Products]
Matching Pairs
Unconjugated Antibody: ATG9B (MBS809760)
Alkaline Phosphatase Conjugated Antibody: ATG9B (MBS809769)
Matching Pairs
Unconjugated Antibody: N/A (MBS809769)
Alkaline Phosphatase Conjugated Antibody: ATG9B (MBS809769)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q674R7
Species Reactivity
Human; Mouse
Specificity
Detects ~100 kDa.
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (lot specific)
Immunogen
Synthetic peptide from the N-terminal of Human ATG9B (aa. 110-121)
Cellular Localization
Cytoplasmic Vesicle; Autophagosome Membrane; Multi-Pass Membrane Protein
Conjugate
Alkaline Phosphatase
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Tissue Specificity
Highly expressed in placenta (trophoblast cells) and pituitary gland. Not expressed in vascular endothelial.
Preparation and Storage
Store at -20 degree C.
Other Notes
Small volumes of anti-ATG9B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ATG9B antibody
ATG9B is involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Highly expressed in the placenta and the pituitary gland.
Product Categories/Family for anti-ATG9B antibody
Antibodies; Cardiovascular System; Heart; Cancer; Autophagy; Cell Signaling; Metabolism; Mitochondrial Metabolism
Applications Tested/Suitable for anti-ATG9B antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-ATG9B antibody
WB: 1:1000
ICC/IF: 1:100
Western Blot (WB) of anti-ATG9B antibody
Western blot analysis of Rat Brain cell lysates showing detection of 101 kDa ATG9B protein using Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 101 kDa.
NCBI/Uniprot data below describe general gene information for ATG9B. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001303985.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001317056.1
[Other Products]
UniProt Primary Accession #
Q674R7
[Other Products]
UniProt Secondary Accession #
Q6JRW5; Q8N8I8; A1A5D3[Other Products]
UniProt Related Accession #
Q674R7[Other Products]
Molecular Weight
36,989 Da
NCBI Official Full Name
autophagy-related protein 9B
NCBI Official Synonym Full Names
autophagy related 9B
NCBI Official Symbol
ATG9B [Similar Products]
NCBI Official Synonym Symbols
SONE; APG9L2; NOS3AS
[Similar Products]
NCBI Protein Information
autophagy-related protein 9B
UniProt Protein Name
Autophagy-related protein 9B
UniProt Synonym Protein Names
APG9-like 2; Nitric oxide synthase 3-overlapping antisense gene protein; Protein sONE
Protein Family
Autophagy-related protein
UniProt Gene Name
ATG9B [Similar Products]
UniProt Synonym Gene Names
APG9L2; NOS3AS; Protein sONE [Similar Products]
NCBI Summary for ATG9B
This gene functions in the regulation of autophagy, a lysosomal degradation pathway. This gene also functions as an antisense transcript in the posttranscriptional regulation of the endothelial nitric oxide synthase 3 gene, which has 3' overlap with this gene on the opposite strand. Mutations in this gene and disruption of the autophagy process have been associated with multiple cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]
UniProt Comments for ATG9B
Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle ().
Research Articles on ATG9B
1. This study highlights the transcriptional inactivation mechanisms of ATG2B, ATG4D, ATG9A and ATG9B promoter methylation status and the possible origin of autophagy signal pathway repression in invasive ductal carcinomas.
Precautions
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Disclaimer
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