Product Name
FLVCR1, siRNA
Full Product Name
FLVCR1 siRNA (Human)
Product Synonym Names
FLVCR; Feline leukemia virus subgroup C receptor-related protein 1; Feline leukemia virus subgroup C receptor; hFLVCR
Product Gene Name
FLVCR1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9Y5Y0
Specificity
FLVCR1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FLVCR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of FLVCR1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
FLVCR1 sirna
siRNA to inhibit FLVCR1 expression using RNA interference
Applications Tested/Suitable for FLVCR1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for FLVCR1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_054772.1
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NCBI GenBank Nucleotide #
NM_014053.3
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UniProt Primary Accession #
Q9Y5Y0
[Other Products]
UniProt Secondary Accession #
Q1HE16; Q86XY9; Q9NVR9[Other Products]
UniProt Related Accession #
Q9Y5Y0[Other Products]
Molecular Weight
30,744 Da
NCBI Official Full Name
feline leukemia virus subgroup C receptor-related protein 1
NCBI Official Synonym Full Names
feline leukemia virus subgroup C cellular receptor 1
NCBI Official Symbol
FLVCR1 [Similar Products]
NCBI Official Synonym Symbols
PCA; AXPC1; FLVCR; PCARP; MFSD7B
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NCBI Protein Information
feline leukemia virus subgroup C receptor-related protein 1
UniProt Protein Name
Feline leukemia virus subgroup C receptor-related protein 1
Protein Family
Feline leukemia virus subgroup C receptor-related protein
UniProt Gene Name
FLVCR1 [Similar Products]
UniProt Synonym Gene Names
FLVCR; Feline leukemia virus subgroup C receptor; hFLVCR [Similar Products]
UniProt Entry Name
FLVC1_HUMAN
NCBI Summary for FLVCR1
This gene encodes a member of the major facilitator superfamily of transporter proteins. The encoded protein is a heme transporter that may play a critical role in erythropoiesis by protecting developing erythroid cells from heme toxicity. This gene may play a role in posterior column ataxia with retinitis pigmentosa and the hematological disorder Diamond-Blackfan syndrome. [provided by RefSeq, Jan 2011]
UniProt Comments for FLVCR1
FLVCR1: Heme transporter that exports cytoplasmic heme. It can also export coproporphyrin and protoporphyrin IX, which are both intermediate products in the heme biosynthetic pathway. Does not export bilirubin. Heme export depends on the presence of HPX and may be required to protect developing erythroid cells from heme toxicity. Heme export also provides protection from heme or ferrous iron toxicities in liver and brain. Causes susceptibility to FeLV-C in vitro. Defects in FLVCR1 are the cause of posterior column ataxia with retinitis pigmentosa (PCARP). A neurodegenerative syndrome beginning in infancy with areflexia and retinitis pigmentosa. Nyctalopia (night blindness) and peripheral visual field loss are usually evident during late childhood or teenage years, with subsequent progressive constriction of the visual fields and loss of central retinal function over time. A sensory ataxia caused by degeneration of the posterior columns of the spinal cord results in a loss of proprioceptive sensation that is clinically evident in the second decade of life and gradually progresses. Scoliosis, camptodactyly, achalasia, gastrointestinal dysmotility, and a sensory peripheral neuropathy are variable features of the disease. Affected individuals have no clinical or radiological evidence of cerebral or cerebellar involvement. Defective neuronal heme transmembrane export due to FLVCR1 mutations may abrogate the neuroprotective effects of neuroglobin and initiate an apoptotic cascade that results in the selective degeneration of photoreceptors in the neurosensory retina and sensory neurons in the posterior spinal cord. Belongs to the major facilitator superfamily. Feline leukemia virus subgroup C receptor (TC 2.A.1.28.1) family.
Protein type: Transporter, iron; Membrane protein, multi-pass; Transporter, ion channel; Transporter; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q32.3
Cellular Component: mitochondrion; integral to plasma membrane; mitochondrial membrane; plasma membrane
Molecular Function: protein binding; transporter activity; heme transporter activity
Biological Process: spleen development; blood vessel development; heme transport; erythrocyte maturation; in utero embryonic development; cellular iron ion homeostasis; multicellular organism growth; multicellular organismal development; regulation of organ growth; mitochondrial transport; embryonic skeletal morphogenesis; transport; erythrocyte differentiation; embryonic digit morphogenesis; transmembrane transport
Disease: Posterior Column Ataxia With Retinitis Pigmentosa
Research Articles on FLVCR1
1. both HIF2alpha and ETS1 are involved in the transcriptional regulation of Flvcr1a and that HIF2alpha is absolutely required for Flvcr1a induction upon hypoxia
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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