Product Name
Prolyl 3-hydroxylase 1 (LEPRE1), Recombinant Protein
Full Product Name
Recombinant Human Prolyl 3-hydroxylase 1 (LEPRE1)
Product Gene Name
LEPRE1 recombinant protein
[Similar Products]
Product Synonym Gene Name
LEPRE1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
23-736aa, Full Length of Mature Protein
Sequence
EVESEAGWGM VTPDLLFAEG TAAYARGDWP GVVLSMERAL RSRAALRALR LRCRTQCAAD FPWELDPDWS PSPAQASGAA ALRDLSFFGG LLRRAACLRR CLGPPAAHSL SEEMELEFRK RSPYNYLQVA YFKINKLEKA VAAAHTFFVG NPEHMEMQQN LDYYQTMSGV KEADFKDLET QPHMQEFRLG VRLYSEEQPQ EAVPHLEAAL QEYFVAYEEC RALCEGPYDY DGYNYLEYNA DLFQAITDHY IQVLNCKQNC VTELASHPSR EKPFEDFLPS HYNYLQFAYY NIGNYTQAVE CAKTYLLFFP NDEVMNQNLA YYAAMLGEEH TRSIGPRESA KEYRQRSLLE KELLFFAYDV FGIPFVDPDS WTPEEVIPKR LQEKQKSERE TAVRISQEIG NLMKEIETLV EEKTKESLDV SRLTREGGPL LYEGISLTMN SKLLNGSQRV VMDGVISDHE CQELQRLTNV AATSGDGYRG QTSPHTPNEK FYGVTVFKAL KLGQEGKVPL QSAHLYYNVT EKVRRIMESY FRLDTPLYFS YSHLVCRTAI EEVQAERKDD SHPVHVDNCI LNAETLVCVK EPPAYTFRDY SAILYLNGDF DGGNFYFTEL DAKTVTAEVQ PQCGRAVGFS SGTENPHGVK AVTRGQRCAI ALWFTLDPRH SERDRVQADD LVKMLFSPEE MDLSQEQPLD AQQGPPEPAQ ESLSGSESKP KDEL
3D Structure
ModBase 3D Structure for Q32P28
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of LEPRE1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
LEPRE1 recombinant protein
This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Two alternatively spliced transcript variants encoding different isoforms have been described. Other variants may exist, but their
biological validity has not been determined.
NCBI/Uniprot data below describe general gene information for LEPRE1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001139761.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001146289.1
[Other Products]
UniProt Primary Accession #
Q32P28
[Other Products]
UniProt Secondary Accession #
Q7KZR4; Q96BR8; Q96SK8; Q96SL5; Q96SN3; Q9H6K3; Q9HC86; Q9HC87[Other Products]
UniProt Related Accession #
Q32P28[Other Products]
Molecular Weight
78,922 Da
NCBI Official Full Name
prolyl 3-hydroxylase 1 isoform 2
NCBI Official Synonym Full Names
prolyl 3-hydroxylase 1
NCBI Official Symbol
P3H1 [Similar Products]
NCBI Official Synonym Symbols
OI8; GROS1; LEPRE1
[Similar Products]
NCBI Protein Information
prolyl 3-hydroxylase 1
UniProt Protein Name
Prolyl 3-hydroxylase 1
UniProt Synonym Protein Names
Growth suppressor 1
UniProt Gene Name
P3H1 [Similar Products]
UniProt Synonym Gene Names
Leprecan-1 [Similar Products]
NCBI Summary for LEPRE1
This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Three alternatively spliced transcript variants encoding different isoforms have been described. Other variants may exist, but their biological validity has not been determined. [provided by RefSeq, Aug 2011]
UniProt Comments for LEPRE1
Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts.
Research Articles on LEPRE1
1. P3H1 mutation is associated with Non-Lethal Type VIII Osteogenesis Imperfecta.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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