7-Alpha-Hydroxycholest-4-en-3-one 12-Alpha-Hydroxylase (CYP8B1), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of CYP8B1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9365844 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 7-Alpha-Hydroxycholest-4-en-3-one 12-Alpha-Hydroxylase (CYP8B1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP8B1. The ELISA analytical biochemical technique of the MBS9365844 kit is based on CYP8B1 antibody-CYP8B1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP8B1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP8B1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

58,068 Da

cytochrome P450 family 8 subfamily B member 1

7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase

7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase

CYP12  [Similar Products]

CP8B1_HUMAN

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless. [provided by RefSeq, Jul 2008]

CYP8B1: Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha- hydroxylated C27 steroids. Belongs to the cytochrome P450 family.

Protein type: Membrane protein, integral; Oxidoreductase; Lipid Metabolism - primary bile acid biosynthesis; EC 1.14.13.95

Chromosomal Location of Human Ortholog: 3p22.1

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: heme binding; iron ion binding; oxygen binding; sterol 12-alpha-hydroxylase activity

Biological Process: bile acid biosynthetic process; bile acid metabolic process; sterol metabolic process; xenobiotic metabolic process

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