ARFGAP1, Monoclonal Antibody

Monoclonal ARFGAP1; Anti-ARFGAP1; ADP ribosylation factor GTPase activating protein; ADP ribosylation factor GTPase activating protein 1; ADP-ribosylation factor 1 GTPase-activating protein; ARF GAP 1; FLJ10767; GAP protein; ARFGAP1; ARFGAP 1; ARFGAP-1

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

1H6

Mouse

ARFGAP1 antibody was purified by affinity chromatography.

Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.

500 ug-1 mg/ml (lot specific)

Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Small volumes of anti-ARFGAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mouse monoclonal ARFGAP1 antibody

Cell Biology

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)

FC: 1:100
IF: 1:100
WB: 1:500-2000

Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ARFGAP1 protein (Right) or empty vector (Left) detected with ARFGAP1 antibody

Immunofluorescent staining of COS7 cells transiently transfected with recombinant ARFGAP1 protein using ARFGAP1 antibody

39,705 Da

ADP-ribosylation factor GTPase activating protein 1

ADP-ribosylation factor GTPase-activating protein 1

ADP-ribosylation factor GTPase-activating protein 1

ARF1GAP; ARF GAP 1; ARF1 GAP  [Similar Products]

ARFG1_HUMAN

The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

ARF GAP1: GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; GAPs, ARF; GAPs

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: synapse; cytosol

Molecular Function: protein binding; zinc ion binding; GTPase activator activity

Biological Process: protein transport; COPI coating of Golgi vesicle; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; unfolded protein response; retrograde vesicle-mediated transport, Golgi to ER; positive regulation of GTPase activity

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