ARFGAP1, Polyclonal Antibody

ARF1GAP; HRIHFB2281

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous levels of total ARFGAP1 protein.

Antigen affinity purification.

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

1.2 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-ARFGAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene.

Total protein Ab

Western Blot (WB), Immunohistochemistry (IHC)

Western blotting: 1:1000-1:5000
Immunohistochemistry: 1:25-1:100

Gel: 10%SDS-PAGE Lysates (from left to right): 293T, A549, PC3 and NIH/3T3 cell Amount of lysate: 40ug per lane Primary antibody: 1/600 dilution Secondary antibody dilution: 1/8000 Exposure time: 10 seconds

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using at dilution 1/30.

39,705 Da

ADP-ribosylation factor GTPase activating protein 1

ADP-ribosylation factor GTPase-activating protein 1

ADP-ribosylation factor GTPase-activating protein 1

ARF1GAP; ARF GAP 1; ARF1 GAP  [Similar Products]

ARFG1_HUMAN

The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

ARF GAP1: GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; Motility/polarity/chemotaxis; GAPs, ARF

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: synapse; cytosol

Molecular Function: protein binding; zinc ion binding; GTPase activator activity

Biological Process: protein transport; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; COPI coating of Golgi vesicle; unfolded protein response; retrograde vesicle-mediated transport, Golgi to ER; positive regulation of GTPase activity

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