产品资料
  首页 >>> 产品目录 >>> 试剂 >>> abm

CHOPWT9 iPSC

如果您对该产品感兴趣的话,可以
产品名称: CHOPWT9 iPSC
产品型号:
产品展商: 其他品牌
产品文档: 无相关文档

简单介绍

CHOPWT9 iPSC


CHOPWT9 iPSC  的详细介绍
Print Version
Safety BioSafety Level II
Organism Human
Donor Female
Source Organ Peripheral Blood Mononuclear Cells
Growth Properties Adherent
Morphology Polygonal
Markers Oct4, Sox2, Nanog, Rex1, DMNT3B, and ABCG2
Picture
Description The CHOPWT9 IPSCs were established by Sendai viral infection carrying reprogramming factors Oct3/4, Sox2, c-Myc, and Klf-4 of peripheral blood mononuclear cells collected from a healthy ***** female. These cells are capable of in vivo tetratoma formation as tested on NOD/SCID mice. The CHOPWT9 IPSCs are valuable in the field of developmental biology as they are useful as a control for applications such as differentiation analyses to the three germ layers and derivative tissues.
Quality Control 1) Flow cytometry analysis of pluripotency markers;
2) Karyotype analysis of chromosomal G-band;
3) qPCR of pluripotency genes
Concentration 106cells/vial
Propagation The iPSCs requires a co-culture with mouse embryonic fibroblasts (MEFs) to proliferate. The base medium for this cell line is Prigrow IV medium available from abm (TM004). To make the completed growth medium, add the following components to the base medium: KnockOutTM Serum Replacement (Invitrogen; A3181502) to final concentration of 15%, 1% NEAA (Invitrogen; 11140050), 1% L-glutamine (G275), 0.1 mM 2-mercaptoethanol (Invitrogen; 21103-049) and 1% Penicillin/Streptomycin Solution (G255). Filter medium with 0.45 µM, before adding 5-10 ng/ml human bFGF (Z101455).Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C.

Add 10 µM ROCK inhibitor (R&D Systems; 1254/50) to media when plating iPSCs on MEFs.
Storage -180°C
Shipping On Dry Ice
Notes For Research Use Only, not for therapeutic or diagnostic purposes.
产品留言
标题
联系人
联系电话
内容
验证码
点击换一张
注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!
相关产品
abmgood|G499
abmgood|G463
abmgood|G232
abmgood|G237
abmgood| G902
abmgood|G905
abmgood|E002
abmgood|2X PCR Taq Plus MasterMix
abmgood|TaqFast DNA Polymerase
abmgood|2X PCR Taq Plus MasterMix with dye
abmgood|2X PCR TaqFast MasterMix
abmgood|2X PCR TaqFast MasterMix with dye
abmgood|Bestaq ; DNA Polymerase
abmgood|2X PCR Bestaq ; MasterMix
abmgood|2X PCR Bestaq ; MasterMix with dye
abmgood|Kodaq DNA Polymerase
abmgood|Kodaq 2X PCR MasterMix
abmgood|Kodaq 2X PCR MasterMix with Dye
abmgood|Bloodirect DNA Polymerase
abmgood|Bloodirect 2X PCR MasterMix
abmgood|Tissue Ex-Amp PCR Kit
abmgood|Plant Ex-Amp PCR Kit
abmgood|PCR-Sure ; Kit
abmgood|SafeView Plus™
abmgood|Safeview FireRed
abmgood|SafeView™
abmgood|OneScript ; Reverse Transcriptase
abmgood|OneScript Plus ; Reverse Transcriptase
abmgood|5X All-In-One RT MasterMix
abmgood|5X All-In-One MasterMix (with AccuRT Genomic DNA Removal Kit)
abmgood|miRNA cDNA Synthesis Kit, with Poly(A) Polymerase Tailing
abmgood|Total-Transcriptome cDNA Synthesis Kit
abmgood|EvaGreen 2X qPCR MasterMix-iCycler
abmgood|EvaGreen 2X qPCR MasterMix-Low ROX
abmgood|EvaGreen 2X qPCR MasterMix-no DYE
abmgood|EvaGreen Express 2X qPCR MasterMix-ROX
abmgood|EvaGreen 2X qPCR MasterMix-ROX
abmgood|EvaGreen Express 2X qPCR MasterMix-Low ROX
abmgood|EvaGreen Express 2X qPCR MasterMix-iCycler
abmgood|EvaGreen Express 2X qPCR MasterMix-No Dye
abmgood|EvaGreen miRNA qPCR Mastermix-iCycler
abmgood|EvaGreen miRNA qPCR Mastermix-low ROX
abmgood|EvaGreen miRNA qPCR Mastermix-ROX
abmgood|EvaGreen miRNA qPCR Mastermix-no dye
abmgood|TaqProbe 2X qPCR MasterMix-ROX
abmgood|TaqProbe 2X qPCR MasterMix-iCycler
abmgood|TaqProbe 2X qPCR MasterMix-Low ROX
abmgood|TaqProbe 2X qPCR MasterMix-Multiplex
abmgood|TaqProbe 2X qPCR MasterMix-No Dye
ABMgood 授权签约代理

沪公网安备 31011202007343号