Catalogue number
CYT-210
Synonyms
MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.
Introduction
IL3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
Description
Interleukin-3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. The IL-3 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a concentrated (1mg/ml) solution in water containing 0.3 mg/ml of NaHCO3.
Solubility
It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Purity
Greater than 98.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Thr-Gln.
Biological Activity
The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 10,000,000 IU/mg.
Protein content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.84 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard.
References
1.
Title:Interleukin-10 overexpression in macrophages suppresses atherosclerosis in hyperlipidemic mice.
Publication:Published online before print March 30, 2010, doi: 10.1096/fj.09-148155
August 2010 The FASEB Journal vol. 24 no. 8 2869-2880 .
Link:http://www.fasebj.org/content/24/8/2869.full
2.Title:A Method for the Selection of Human Embryonic Stem Cell Sublines with High Replating Efficiency After Single-Cell Dissociation.
Publication:Article first published online: 24 AUG 2006 DOI:10.1634/stemcells.2005-0657 Copyright © 2006 AlphaMed Press
Link:http://onlinelibrary.wiley.com/doi/10.1634/stemcells.2005-0657/full
3.Title: Selective Activation of Human Dendritic
Cells by OM-85 through a NF-kB and MAPK Dependent Pathway
Publications: PloS one 8.12 (2013): e82867
Link: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0082867#pone-0082867-g005
4. Title: Novel markers of normal and neoplastic
human plasmacytoid dendritic cells
Publication: Blood 111.7 (2008): 3778-3792.
Link: http://www.bloodjournal.org/content/111/7/3778
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
