Catalogue number
CYT-685
Synonyms
Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.
Introduction
Transforming growth factor betas (TGFBetas) mediate many cell-cell interactions that occur during embryonic development. Three TGFBetas have been identified in mammals. TGFBeta1, TGFBeta2 and TGFBeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.
Description
TGF-b 3 Human Recombinant produced in CHO is a disulfide-linked homodimeric, glycosylated, polypeptide chain containing 112 amino acids and having a molecular mass of 25kDa.
The TGF-b 3 is purified by standard chromatographic techniques.
Source
Chinese Hamster Ovarian Cells.
Physical Appearance
Sterile Filtered clear solution.
Formulation
The protein solution contains 20% Ethanol and 0.1% Acetic acid.
Stability
TGF-beta 3 although stable at 4°C. for 3 weeks, should be stored at -20°C to -70°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Purity
Greater than 97.0% as determined by SDS-PAGE.
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Asp-Thr-Asn.
Biological Activity
The
biological activity was determined using cell toxicity assay via HT-2 cells. The ED50 was found to be < 0.05ng/ml, corresponding to a specific activity of 20,000,000IU/mg.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
