FKBP14 Human

FKBP22, FKBP-14, FK506 Binding Protein 14, FKBP14, EC=5.2.1.8, PPlase FKBP14, Peptidyl-prolyl cis-trans isomerase FKBP14, FLJ20731.

FKBP14 enzyme accelerates the folding of proteins during protein synthesis. FKBP14 contains 2 EF-hand domains and one PPIase FKBP-type domain. Truncation of the amino-terminus of FKBP14 significantly decreases peptidyl prolyl cis-trans isomerase activity, therefore implicating that the PPIase FKBP-type domain must be located at the N-terminus.

FKBP14 Recombinant E.coli produced in E.Coli is a single, non-glycosylated polypeptide chain containing 213 amino acids (20-211 a.a.) and having a molecular mass of 24.2 kDa. The FKBP14 is fused to 21 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile filtered colorless solution.

FKBP14 Human (1mg/ml) solution containing 1x PBS pH-7.4, & 10% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Greater than 90.0% as determined by SDS-PAGE.

Specific activity is > 240 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25°C in Tris-HCl pH8.0 using chymotrypsin.

MGSSHHHHHH SSGLVPRGSH MALIPEPEVK IEVLQKPFIC HRKTKGGDLM LVHYEGYLEK DGSLFHSTHK HNNGQPIWFT LGILEALKGW DQGLKGMCVG EKRKLIIPPA LGYGKEGKGK IPPESTLIFN IDLLEIRNGP RSHESFQEMD LNDDWKLSKD EVKAYLKKEF EKHGAVVNES HHDALVEDIF DKEDEDKDGF ISAREFTYKH DEL.

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