Catalogue number
ENZ-823
Synonyms
RNA Guanylyltransferase And 5'-Phosphatase, CAP1A, RNA
Guanylyltransferase And 5-Phosphatase, HCAP1, HCE1, HCE, MRNA-Capping Enzyme, HCAP
3, mRNA-capping enzyme.
Introduction
RNA Guanylyltransferase And 5'-Phosphatase also known as RNGTT is a
bifunctional mRNA-capping enzyme which exhibits RNA 5'-triphosphatase activity
in the N-terminal section and mRNA guanylyltransferase activity in the
C-terminal section. In addition, RNGTT
catalyzes the first two steps of cap formation, through removing the
gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a
diphosphate end, and also by transferring the gmp moiety of GTP to the
5'-diphosphate terminus.
Description
RNGTT Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 620 amino acids (1-597a.a) and
having a molecular mass of 70.9kDa.
RNGTT is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
RNGTT protein solution (0.25mg/ml) containing 20mM
Tris-HCl buffer (pH 7.5), 0.2M NaCl, 40% glycerol, 2mM DTT and 0.1mM PMSF.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAHNKIP PRWLNCPRRG QPVAGRFLPL KTMLGPRYDS QVAEENRFHP
SMLSNYLKSL KVKMGLLVDL TNTSRFYDRN DIEKEGIKYI KLQCKGHGEC PTTENTETFI RLCERFNERN
PPELIGVHCT HGFNRTGFLI CAFLVEKMDW SIEAAVATFA
QARPPGIYKG DYLKELFRRY GDIEEAPPPP LLPDWCFEDD EDEDEDEDGK
KESEPGSSAS FGKRRKERLK LGAIFLEGVT VKGVTQVTTQ PKLGEVQQKC HQFCGWEGSG FPGAQPVSMD
KQNIKLLDLK PYKVSWKADG TRYMMLIDGT NEVFMIDRDN SVFHVSNLEF PFRKDLRMHL
SNTLLDGEMI IDRVNGQAVP RYLIYDIIKF NSQPVGDCDF NVRLQCIERE
IISPRHEKMK TGLIDKTQEP FSVRNKPFFD ICTSRKLLEG NFAKEVSHEM DGLIFQPTGK YKPGRCDDIL
KWKPPSLNSV DFRLKITRMG GEGLLPQNVG LLYVGGYERP FAQIKVTKEL KQYDNKIIEC
KFENNSWVFM RQRTDKSFPN AYNTAMAVCN SISNPVTKEM LFEFIDRCTA
ASQGQKRKHH LDPDTELMPP PPPKRPRPLT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
