Dengue Epitope 10

Caused by one of four closely related virus serotypes of the genus Flavivirus, family Flaviviridae, each serotype is sufficiently different that there is no cross-protection and epidemics caused by multiple serotypes (hyperendemicity) can occur. In cell culture experiments and mice Morpholino antisense oligos have shown specific activity against Dengue virus.

Recombinant Dengue Multiple Epitopes 10 is genetically designed dengue multiple epitopes epi-10 designed especially for lateral flow product, they are selected from dengue genome. The rapid test prepared by this antigen has over 90% sensitivity and specificity of over 90% to show quick and strong signal for both dengue IgM and IgG. Conjugate gold suspension buffer, blocking reagent and running buffer den- epi-10 comes with the order.

Escherichia Coli.

Protein is >95% pure as determined by 12% PAGE (coomassie staining).

Phosphate buffered saline, pH 7.4 and 0.02% sodium nitrate.

1. Prepare 526-532nm colloid gold, conjugation within 24 hours after colloid gold preparation.
2. Adjust colloid gold OD at 1.3-1.4, conjugation concentration and pH are 16µg/ml gold and 7.2.
3. Shanking conjugation for 8-10 hours, then centrifuge the conjugation at 10,000 rpm at 20^oC for 20 min.
4. Remove the supernatnant as more as possible, add the supplied gold suspension buffer to adjust OD to 8.2.
5. Add the supplied blocking reagent to the final concentration to 0.8%.
6. Blocking for 1 hour, then add sucrose to the final concentration to 10 to 12%.
7. Dipping the conjugated gold to the gold pad (do not use sodium phosphate for gold pad treatment).
8. After drying, assemble gold pad, the sample padand absorbent pad over backing card.
9. Add 10µl serum/plasma and add the supplied epi-10 running buffer for assay.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.