Catalogue number
HEV-273
Introduction
Hepatitis E virus (HEV), the major etiologic agent of enterically transmitted non-A, non-B hepatitis worldwide, is a spherical, non-enveloped, single stranded RNA virus that is approximately 32 to 34 nm in diameter. HEV belongs to a genus of HEV-like viruses (unassigned genus). HEV has a single-stranded polyadenylated RNA genome of approximately 8 kb. Based on its physicochemical properties it is presumed to be a calici-like virus.
Description
The e.coli derived HEV protein is fused with beta-galactosidase at the N-Terminus and contains the HEV immunodominant ORF3 92-123 a.a.
Purification Method
HEV ORF3 protein was purified by proprietary chromatographic technique.
Purity
HEV ORF3 protein is >95% pure as determined by 10% PAGE (coomassie staining).
Formulation
20mM Tris-HCl, pH-8, 10mM B-ME and 8M urea.
Stability
HEV ORF3 protein although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.
Specificity
Immunoreactive with sera HEV-infected individuals.
Applications
HEV ORF3 antigen is suitable for ELISA and Western blots, excellent antigen for detection of HEV with minimal specificity problems.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY.They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
