BolA-like protein 1, hBolA, BOLA1, CGI-143.
BolA Homolog 1(BOLA1) is a member of the bolA/yrbA family. BolA proteins, which include the: hBolA1, hBolA2, and hBolA3, are novel non-classical secreted proteins detected with bioinformatics and molecular
biology experiments. The 3 BolA fusion proteins with c-Myc tag can be secreted into the culture medium of the transfected Cos-7 cells, even though they could not be colocalized with Golgi apparatus. Also, the secretion of 3 BolA proteins could not be inhibited after BFA treatment.
BOLA1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 140 amino acids (21-137 a.a) and having a molecular mass of 14kDa.
BOLA1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Escherichia Coli.
Sterile Filtered colorless solution.
BOLA1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 20% glycerol and 1mM DTT.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Greater than 95.0% as determined by SDS-PAGE.
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