Wnt
inhibitory factor 1, WIF-1, Wif1.
WIF1 produced in Sf9 Insect cells is a single,
glycosylated polypeptide chain containing 359 amino acids (29-379a.a.) and
having a molecular mass of 39.4kDa. (Molecular size on SDS-PAGE will appear at
approximately 40-57kDa).
WIF1 is expressed with an 8 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Sf9,
Insect cells.
Sterile
filtered colorless solution.
WIF1
protein solution (1mg/ml) contains 20mM MES (pH5.5), 1mM DTT, 1mM PMSF and 30%
glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Greater than 90.0% as
determined by SDS-PAGE.
GQPPEESLYL WIDAHQARVL IGFEEDILIV SEGKMAPFTH DFRKAQQRMP
AIPVNIHSMN FTWQAAGQAE YFYEFLSLRS LDKGIMADPT VNVPLLGTVP HKASVVQVGF PCLGKQDGVA
AFEVNVIVMN SEGNTILRTP QNAIFFKTCQ QAECPGGCRN GGFCNERRVC ECPDGFYGPH
CEKALCIPRC MNGGLCVTPG
FCICPPGFYG VNCDKANCST TCFNGGTCFY PGKCICPPGL EGEQCELSKC PQPCRNGGKC IGKSKCKCPK GYQGDLCSKP
VCEPGCGAHG TCHEPNKCQC REGWHGRHCN KRYGASLMHA PRPAGAGLER HTPSLKKAED RRDPPESNYI WVEHHHHHH.
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
