GABA-A receptor α1 - 224 204
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产品名称: GABA-A receptor α1 - 224 204
产品型号: 224 204
产品展商: 其他品牌
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简单介绍
GABA-A receptor α1 - 224 204
GABA-A receptor α1 - 224 204
的详细介绍
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Cat. No. 224 204
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100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
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Applications
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WB: 1 : 1000 up to 1 : 2000 (AP staining) (see remarks) gallery
IP: not tested yet
ICC: 1 : 200 up to 1 : 1000 (see remarks) gallery
IHC: 1 : 500 (see remarks) gallery
IHC-P/FFPE: not tested yet
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Immunogen
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Synthetic peptide corresponding to AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813)
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Reactivity
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Reacts with: rat (P62813), mouse (P62812).
Other species not tested yet.
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Specificity
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Specific for GABA-A receptor α1.
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matching control protein/peptide
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224-2P
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Remarks
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WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondaryreagents. IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).
recommended protocol
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Datasheet
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224_204.pdf
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