dCas9 C-terminal Cloning Vector
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产品名称: dCas9 C-terminal Cloning Vector
产品型号: K014
产品展商: 其他品牌
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简单介绍
dCas9 C-terminal Cloning Vector
dCas9 C-terminal Cloning Vector
的详细介绍
|
Print Version |
| Description |
This Cas9 Double Mutant (dCas9) has changes at amino acid positions D10A and H840A which completely inactivate both the nuclease and nickase activities. With a target-specific guide RNA (sgRNA), it can bind to a specific region of DNA without cutting it. This vector provides an in-frame downstream multiple cloning site, allowing users to attach any protein of interest to the C-terminus of dCas9. The resulting construct will also carry a C-terminal nuclear localization signal. |
| Product Application |
Direct non-viral plasmid transfection for immediate expression
Package into Lentiviral particles for high efficiency transduction and stably integrated expression |
| Species |
N/A |
| Vector |
pLenti-EF1a-dCas9-Cloning |
| Bacterial Selection |
Kanamycin |
| Format |
Plasmid |
| Appearance |
Liquid |
| Storage |
-20°C or below |
| Shelf Life |
1 year (when at -20°C or below in a non-frost free freezer) |
| Shipping |
Shipped at ambient temperature |
| Additional Materials |
- CRISPR Genomic Cleavage Detection Kit (G932)
- sgRNA CRISPR Lentivector Control (K018) |
| Quality Control |
Restriction Enzyme Digest and Sequencing |
| Caution |
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
| Note |
abm's sgRNAs are designed for use with Cas9 Nuclease only. Please inquire for custom sgRNAs for purposes other than genome editing (e.g. CRISPRa). For CRISPR-Cas9 system applications in genome regulation, please see our comprehensive collection of Cas9 mutant proteins (Nickase, Null mutant). |
| Disclaimer |
1. The end user acknowledges that the Materials provided under abm’s Material Transfer Agreement (MTA) does not grant a license for commercial use or imply any ownership rights of any intellectual property rights relating to the Materials. |
